Combinatorial chemistry technology: method – library – apparatus – Apparatus specially adapted for use in combinatorial... – Integrated apparatus specially adapted for both screening a...
Reexamination Certificate
2005-05-05
2010-06-29
Steele, Amber D. (Department: 1639)
Combinatorial chemistry technology: method, library, apparatus
Apparatus specially adapted for use in combinatorial...
Integrated apparatus specially adapted for both screening a...
C436S518000, C548S152000, C548S217000, C548S452000
Reexamination Certificate
active
07745377
ABSTRACT:
The invention provides peptide synthons having protected functional groups for attachment of desired moieties (e.g. functional molecules or probes). Also provided are peptide conjugates prepared from such synthons, and synthon and conjugate preparation methods including procedures for identifying the optimum probe attachment site. Biosensors are provided having environmentally sensitive dyes that can locate specific biomolecules within living cells and detect chemical and physiological changes in those biomolecules as the living cell is moving, metabolizing and reacting to its environment. Methods are included for detecting GTP activation of a Rho GTPase protein using polypeptide biosensors. When the biosensor binds GTP-activated Rho GTPase protein, the environmentally sensitive dye emits a signal of a different lifetime, intensity or wavelength than when not bound. New fluorophores whose fluorescence responds to environmental changes are also provided that have improved detection and attachment properties, and that can be used in living cells, or in vitro.
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Dharmawardhane
Bark Steven J.
Burton Dennis R.
Chamberlain Chester
Hahn Klaus M.
Kraynov Vadim
Chadwick Robin A.
Fitting Thomas
Steele Amber D.
The Scripps Research Institute
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