Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Chemical modification or the reaction product thereof – e.g.,...
Patent
1985-08-29
1988-11-15
Wiseman, Thomas G.
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Chemical modification or the reaction product thereof, e.g.,...
530300, 530307, 530308, 530313, 530316, 530323, 530350, 530388, 530398, 530399, 530403, 530404, 530405, 530406, 435 7, 435199, 435207, 435188, C12N 922, C12N 938, C12N 996, C12Q 100, C07G 700, C07K 1300, G01N 5300
Patent
active
047850809
ABSTRACT:
A highly sensitive, immunoassay method for determining the amount of an analyte in a sample containing a known analyte in an unknown concentration is provided. Sample; a polypeptide-labeled analog of the analyte, an antibody specific for said analyte, a polypeptide partner capable of non-covalently binding with the polypeptide-labeled analyte to form a complex having catalytic activity, and a substrate capable of being converted to a reporter molecule by the catalytic activity of said complex are brought together in a medium. The polypeptide-labeled analyte analog is capable of competitively binding to the antibody and the polypeptide partner, the antibody inhibiting the formation of a catalytically active complex in the absence of analyte, and the concentration of the antibody, polypeptide partner and polypeptide-labeled analyte are such as to cause varying amounts of analyte to be directly related to the conversion of the substrate to the reporter molecule. Conversion of the substrate to the reporter molecule is then determined, and compared to conversions of substrate to reporter molecule obtained with known concentrations of the analyte.
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Farina Peter R.
Golke James R.
Baker Instruments Corporation
Rauchfuss, Jr. George W.
Teskin Robin Lyn
Wiseman Thomas G.
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