L5 shuttle phasmids

Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses

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4353201, C12N 1564, C12N 1574

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active

057503842

ABSTRACT:
This invention is directed to L5 shuttle phasmids capable of delivering foreign DNA into mycobacteria and to methods of producing L5 shuttle phasmids. In addition, this invention is directed to a method of generating mycobacterial mutations and to a method of producing mycobacterial vaccines.

REFERENCES:
patent: 4861709 (1989-08-01), Ulitzer et al.
patent: 5504005 (1996-04-01), Bloom et al.
Graham F. Hatfull et al., entitled "DNA sequence, structure and gene expression of mycobacteriophage L5: a phage system for mycobacterial genetics," Molecular Microbiology, pp. 395-405 (1993).
Lee et al. (1991) Site-specific integration of mycobacteriophage L5: integration-proficient vectors for Mycobacterium smegmatis, Mycobacterium tuberculosis, and bacille Calmette-Guerin. Proc. Natl. Acad. Sci. USA 88:3111-3115, Apr. 1991.
Snapper et al. (1988) Lysogeny and transformation in mycobacteria: stable expression of foreign genes. Proc. Natl. Acad. Sci. USA 85:6987-6991, Sep. 1988.

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