Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Oxidoreductase
Patent
1997-10-02
1999-01-19
Wax, Robert A.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Oxidoreductase
435189, 4352523, 43525233, 4353201, 435822, 536 232, C12N 904, C12N 120, C12N 1500, C07H 2104
Patent
active
058612920
ABSTRACT:
A novel L-sorbose dehydrogenase (SDH) and a novel L-sorbosone dehydrogenase (SNDH) both derived from Gluconobacter oxydans T-100, a DNA which encodes the SDH and/or SNDH, an expression vector which contains the DNA, a host cell transformed by the expression vector and a process for producing the SDH and/or SNDH, which comprises culturing the host cell in a medium and recovering the SDH and/or SNDH from the resulting culture. The SDH and SNDH of the present invention are useful enzymes having preferable properties for the production of 2-keto-L-gulonic acid, as well as L-ascorbic acid. According to the production method of the present invention, the SDH and SNDH having such preferable properties can be produced in large amounts by genetic engineering.
Ishii Yoshinori
Niwa Mineo
Saito Yoshimasa
Suzuki Hiromi
Yoshida Masaru
Fujisawa Pharmaceutical Co. Ltd.
Slobodyansky Elizabeth
Wax Robert A.
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