L-ribose isomerase, its preparation and uses

Details L-ribose isomerase, its preparation and uses L-ribose isomerase, its preparation and uses

2000-05-31

2001-09-25

Prats, Francisco (Department: 1651)

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Isomerase

C435S252100, C435S094000, C435S105000, C536S001110, C536S125000

Reexamination Certificate

active

06294369

ABSTRACT:

BACKGROUND OF THE INVENTION
1. Field of the Invention
The present invention relates to an L-ribose isomerase, its preparation and uses, more particularly, relates to an L-ribose isomerase which converts L-ribose into L-ribulose and vice versa, preparation thereof, microorganisms capable of producing the L-ribose isomerase, and a process for producing ketoses and aldoses using the L-ribose isomerase.
2. Description of the Prior Art
Biochemical industries have been developing in these days, and rare saccharides which had been put aside are in great demand in the field of saccharide chemistry. Thus, the establishment of these rare saccharides is strongly required. Although such rare saccharides can be produced by organic chemical methods, the production conditions are generally crucial and the yields of desired products are relatively low. Therefore, the organic chemical methods are not satisfactory as an industrial scale production. While enzymatic saccharide-conversion methods may be imagined as biochemical methods for producing rare saccharides but there was reported no isomerase, which acts on L-ribose or D-talose as a rare saccharide, and was not established the production method for such rare saccharides.
SUMMARY OF THE INVENTION
It has been strongly required an industrial-scale production method for rare saccharides such as L-ribose and D-talose.
To attain the object the present inventors studied on an L-ribose isomerase and extensively screened microorganisms which produce such an enzyme. As a result, the inventors found that a newly isolated microorganism of the species
Acinetobacter calcoaceticus
LR7C strain, isolated from a soil in Miki-machi, Kita-gun, Kagawa, Japan, produces an L-ribose isomerase. The inventors also found that the L-ribose isomerase facilitates the production of rare saccharides when acts on aldoses or ketoses as substrates and established the present invention.


REFERENCES:
Shimonishi et al, J. Ferment. Bioengineer., 81(6):493-497 (1996).*
Holt et al, Bergey's Manual of Determinative Bacteriology, 9th Ed., pp. 73, 103, 129 (1994).*
“List of Cultures” Institute for Fermentation, Osaka (IFO), 9th Ed., pp. V-VI, 95, 136 (1992).*
Izumori et al, “Purification, Crystallization, and Properties of D-ribose Isomerase from Mycobacterium Smegmatis”, J. Biol. Chem. 250(20):8085-8087 (1975).*
Izumori et al, “Induction of D-ribose Isomerase by L-ribose in Mycobacterium Smegmatis”, Agric. Biol. Chem. 44(1):223-225 (1980).*
Trimur et al, “Isolation and Characterization ofEscherichia coliMutants Able to Utilize the Novel Pentose L-ribose”, J. Bacteriol. 137(8):2459-2464 (1991).

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