L-glutamate oxidase

Details L-glutamate oxidase L-glutamate oxidase

2006-09-19

2006-09-19

Achutamurthy, Ponnathapu (Department: 1652)

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Oxidoreductase

C435S004000, C435S006120, C435S018000, C435S252300, C435S320100, C435S069100, C435S071100, C435S440000, C536S023200, C536S023700

Reexamination Certificate

active

07109008

ABSTRACT:
The present invention provides a novel L-glutamate oxidase, a gene encoding the enzyme, and a method for producing the enzyme. By use of a gene encoding the enzyme, L-glutamate oxidase can be readily prepared at low costs through a recombinant DNA technique. The novel L-glutamate oxidase has the following physicochemical properties:(A) action: catalyzing the following reaction:in-line-formulae description="In-line Formulae" end="lead"?L-glutamic acid+O2+H2O→α-ketoglutaric acid+H2O2+NH3;in-line-formulae description="In-line Formulae" end="tail"?(B) substrate specificity: being specific to L-glutamic acid;(C) molecular weight and subunit structure: molecular weight as determined through SDS-polyacrylamide gel electrophoresis of 70,000±6,000, molecular weight as determined through gel filtration of 140,000±10,000, and being a dimer formed of the same subunits having a molecular weight of 70,000±6,000;(D) optimum pH: around pH 6.0 to 8.5;(E) heat stability: being stable up to 60° C. at a pH of 7.4 for 30 minutes; and(F) coenzyme: flavin adenine dinucleotide (FAD).

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