L-cysteine producing bacterium and method for producing...

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Transferase other than ribonuclease

Reexamination Certificate

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C435S160000, C435S113000, C435S183000, C435S252100, C530S350000, C536S023100

Reexamination Certificate

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07148047

ABSTRACT:
L-cysteine is produced by culturing a coryneform bacterium in which intracellular serine acetyltransferase activity is increased by transformation with a gene coding for serine acetyltransferase, such modification of an expression control sequence of a gene coding for serine acetyltransferase that expression of the gene in a cell should be enhanced and for forth, preferably in which L-cysteine decomposition system is further suppressed, in a medium to produce and accumulate L-cysteine in culture and collecting the L-cysteine from the culture.

REFERENCES:
patent: 2235752 (1997-05-01), None
patent: WO 97/15673 (1997-06-01), None
S. Nakamori, et al., Applied and Environmental Microbiology, vol. 64, No. 5, XP-002115630, pp. 1607-1611, “Overproduction of L-Cysteine and L-Cystine byEscherichia colistrains with a genetically Altered Serine Acetyltransferase”, May 1998.
R. Kraemer, Journal of Biotechnology, vol. 45, No. 1, XP-004036833, pp. 1-21, “Genetic and Physiological Approaches for the production of Amino Acids”, Feb. 12, 1996.
Patent Abstracts of Japan, JP 11-155571, Jun. 15, 1999.
N. M. Kredich, et al., The Journal of Biological Chemistry, vol. 241, No. 21, pp. 4955-4965, “The Enzymic Synthesis of l-cysteine inEscherichia coliandSalmonella typhimurium”, Nov. 10, 1966.
D. Denk, et al., Journal of Gernal Microbiology, vol. 133, pp. 515-525, “L-Cysteine Biosynthesis inEscherichia coli: Nucleotide Sequence and Expression of the Serien Acetyltransferase (cysE) Gene from the Wild-Type and a Cysteine-Excreting Mutant”, 1987.
I. Rossol, et al., Journal of Bacteriology, vol. 174, No. 9, pp. 2968-2977, “The Corynebacterium Glutamicum aecD Gene Encodes a C-S Lyase with α,β-Elimination Activity that Degrades Aminoethylcysteine”, May 1992.

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