KYRT1, a disarmed version of a highly tumorigenic Agrobacterium

Multicellular living organisms and unmodified parts thereof and – Plant – seedling – plant seed – or plant part – per se – Higher plant – seedling – plant seed – or plant part

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

4352522, 4352523, 4353201, 435469, 800278, 800306, 800307, 800312, 800314, 800315, 800317, 8003171, 8003173, 8003174, 8003202, 800322, A01H 500, C12N 121, C12N 514, C12N 1586

Patent

active

059293063

ABSTRACT:
Disarmed A. tumefaciencs strain KYRT1, derived from a highly tumorigenic strain identified as A. tumefaciens strain Chry5. Disarming is accomplished by inactivation of plasmid pTiChry5 T-DNA sequences by, for example, deletion of sequences comprising the T-DNA right border. Methods of making transgenic plants using the novel A. tumefaciens strains are also provided.

REFERENCES:
patent: 5416011 (1995-05-01), Hinchee et al.
Simpson et al. A disarmed binary vector from Agrobacterium tumefaciens functions in Agrobacterium rhizogenes. Plant Molecular Biology. 6:403-415, 1986.
E. Hood et al., "New Agrobacterium helper plasmids for gene transfer to plants," Transgenic Research, 2: 208-218 (1993).
E. Hood et al., "Virulence of Agrobacterium tumefaciens Strain A281 on Legumes," Plant Physiol., 83: 529-534 (1987).
E. Hood et al., "The hypervirulence of Agrobacterium tumefaciens A281 is encoded in a region of pTiBo542 outside of T-DNA," J. Bacteriol., 168: 1291-1301 (1986).
Improved antibiotic-resistance gene cassettes and omega elements for Escherichia coli vector construction and in vitro deletion/insertion mutagenesis, M. Alexeyev et al., Gene, 160(1995)63-67.
Mini-Tn10 transposon derivatives for insertion mutagenesis and gene delivery into the chromosome of Gram-negative bacteria, M. Alexeyev et al., Gene, 160(1995)59-62.
T-DNA of the Agrobacterium TI and RI Plasmids, M. Bevan et al., Ann. Rev. Genet. 1982, 16:357-84.
Characterization of an Unusual New Agrobacterium tumefaciens Strain from Chrysanthemum morifolium Ram, A. Bush et al., Applied and Environmental Microbiology, Sep. 1991, pp. 2468-2472.
Strain and cultivar specificity in the Agrobacterium-soybean interaction, M. Byrne et al., Plant Cell, Tissue and Organ Culture 8:13-15 (1987).
Agrobacterium gene transfer: Progress on a "poor man's vector" for maize, M. Chilton, Proc. Nat'. Acad. Sci. USA, vol. 90, pp. 3119-3120, Apr. 1993.
Transgenic analysis of the 5'--and 3' -flanking regions of the NADH-dependent hydroxypyruvate reductase gene from Cucumix sativus L, S. Daniel et al., Plant Molecular Biology 28: 821-836, 1995.
The Host Range of Crown Gall, M. Clene et al., The Botanical Review, vol. 42, Oct.-Dec. 1976, No. 4, pp. 389-466.
Mini-Ti: A New Vector Strategy for Plant Genetic Engineer, A. de Framond et al., Bio/technology, May 1983, pp. 262-269.
Broad host range DNA cloning system for Gram-negative bacteria: Construction of a gene bank of Rhizobium meliloti, G. Ditta et al., Proc. Natl. Acad. Sci. USA, vol. 77, No. 12, pp. 7347-7351, Dec. 1980.
Production of Agrobacterium-mediated transgenic fertile plants by direct somatic embryogenesis from immature zygotic embryos of Datura innoxia, C. Ducrocq et al., Plant Molecular Biology 25: 995-1009, 1994.
Regulation of Agrobacterium tumefaciens T-cyt gene expression in leaves of transgenic potato (Solanum tuberosum L. cv. Desiree) is strongly influenced by plant culture conditions, D. Dymock et al., Plant Molecular Biology 17: 711-725, 1991.
Identification of unexplained DNA fragments within the T-DNA borders of the Bin 19 plant transformation vector, R. Fray et al., Plant Molecular Biology 25: 339-342, 1994.
Growth Inhibition and Loss of Virulence in Cultures of Agrobacterium tumefaciens Treated with Acetosyringone, C. Fortin et al., Journal of Bacteriology, Sep. 1992, pp. 5676-5685.
Construction of a broad host range cosmic cloning vector and its use in the genetic analysis of Rhizobium mutants, A. Friedman et al., Gene, 18 (1982) pp. 289-296.
An essential virulence pf Agrobacterium tumefaciens, VirB4, requires an intact mononucleotide binding domain to function in transfer of T-DNA, K. Fullner et al., Mol Gen Genet (1994) 245:704-715.
Expression of a Bacterial Phaseolotoxin-Resistant Ornithyl Transcarbamylase in Transgenic Tobacco Confers Resistance to Pseudomonas Syringae pv. Phaseolicola, J. de la Fuente-Martinez et al., Bio/Technology vol. 10, Aug. 1992, pp. 905-909.
DNA Flux Across Genetic Barriers: The Crown Gall Phenomenon, G. Gheysen et al., Plant Gene Research: Genetic Flux in Plants, 1985, pp. 11-47.
Illegitimate recombination in plants: a model for T-DNA integration, G. Gheysen et al., Gene & Development 5:287-297, 1991.
The small, versatile pPZP family of Agrobacterium binary vectors for plant transformation, P. Hajdukiewicz et al., Plant Molecular biology 25:989-994, 1994.
Re-evaluation of Conditions for Plant Regeneration and Agrobacterium-Mediated Transformation from Tomato (Lycopersicon esculentum), S. Hamza et al., Journal of Experimental Botany, vol. 44, No. 269, pp. 1837-1845, 1993.
Optimization of biolistic transformation of embryogenic grape cell suspensions, D. Hebert et al., Plant Cell Reports (1993) 12:585-589.
Agrobacterium and plant genetic engineering, P. Hooykaas et al., Plant Molecular Biology 19: 15-38, 1992.
Transformation of plant cells via Agrobacterium, P. Hooykaas, Plant Molecular Biology 13: 327-336, 1989.
The Virulence System of Agrobacterium Tumefaciens, P. Hooykaas et al., Annu. Rev. Phytopathol. 1994, 32:157-79.
Inheritance of Functional Foreign Gene in Plants, R. Horsch et al., Science, vol. 223, Feb., 1984, pp. 496-498.
A Simple and General Method for Transferring Genes into Plants, R. Horsch et al., Science, vol. 227, Mar. 1985, pp. 1227-1231.
Acetosyringone and osmoprotectants like betaine or proline synergistically enhance Agrobacterium-mediated transformation of apple, D. James et al., Plant Cell Reports (1993) 12:559-563.
Evaluation of a Cotyledonary Node Regeneration System for Agrobacterium-Mediated Transformation of Pea (Pisum Sativum L.), M. Jordan et al.
Genetic Analysis of T-DNA Transcripts in Nopaline Crown Galls, H. Joos et al., Cell. vol. 32, 1057-1067, Apr. 1983.
Molecular Mechanisms of Crown Gall Tumorigensis, C. Kado, Critical Reviews in Plant Sciences, 10(1):1-32(1991).
Mapping and genetic organization of pTiChry5, a novel Ti plasmid from a highly virulent Agrobacterium tumefaciens strain, L. Kovacs et al., Mol. Gen. Genet(1994) 242:327-336.
The Chromosomal Background of Agrobacterium tumefaciens Chry5 Conditions High Virulence on Soybean, L. Kovacs et al., MPMI, vol. 6, No. 5, 1993, pp. 601-608.
The Mechanism of T-DNA Transfer from Agrobacterium tumefaciens to the Plant Cell, Z. Koukolikova-Nicola et al.
Multiple copies of virG enhance the transient transformation of celery, carrot and rice tissues by Agrobacterium tumefaciens, C. Liu et al.,Plant Molecular Biology 20: 1071-1087, 1992.
Agrobacterium induced gall formation in bell pepper (Capsicum annuum L.) and formation of shoot-like structures expressing introduced genes, W. Liu et al., Plant Cell Reports (1990) 9:360-364.
A simple procedure for the expression of genes in transgenic soybean callus tissue, G. Luo et al., Plant Cell Reports (1994) 13:632-636.
Inheritance of Soybean Susceptibility to Agrobacterium tumefaciens and its Relationship to Transformation, A. Mauro et al., Crop Sci. 35:1152-1156 (1995).
Susceptibility of some Brazilian soybean genotypes to three strains of Agrobacterium tumefaciens, A. Mauro et al., Brazilian Journal of Genetics 18, 3, 417-420 (1995).
Physical and Genetic Characterization of Symbiotic and Auxotrophic Mutants of Rhizobium meliloti Induced by Transposon Tn5 Mutagenesis, H. Meade et al., Journal of Bacteriology, Jan. 1982, pp. 114-122.
Plasmids Specifying Plant Hyperplasias, E. Nester, Ann. Rev. Microbiol. 1981, 35:531-65.
Strength and tissue specificity of chimeric promoters derived from the octopine and mannopine synthase genes, M. Ni et al., The Plant Journal (1995), 661-676.
Transformation of Soybean Cells Using Mixed Strains of Agrobacterium tumefaciens and Phenolic Compounds, L. Owens et al., Plant Physiol. (1988) 88, 570-573.
Constitutive Mutations of Agrobacterium tumefaciens Transcriptional Activator virG, G. Pazour et al., Journal of Bacteriology, Jun. 1992, pp. 4169-4174.
T-DNA border sequences required for crown gall tumorigenesis, E. Peralta et al., Proc. Natl. Acad. Sci. USA, vol. 82, pp. 5112-5116, Aug. 1985.
Petunia plants escape from negative selection against a transgene by silencing the foreign DNA via methylat

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

KYRT1, a disarmed version of a highly tumorigenic Agrobacterium does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with KYRT1, a disarmed version of a highly tumorigenic Agrobacterium , we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and KYRT1, a disarmed version of a highly tumorigenic Agrobacterium will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-880500

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.