Kunitz domain polypeptide zkun6

Chemistry: molecular biology and microbiology – Vector – per se

Reexamination Certificate

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Details

C435S069100, C435S325000, C530S300000, C530S321000, C530S350000, C536S001110, C536S018700, C536S022100, C536S023100, C536S023400, C536S023500, C536S024100, C536S024200, C536S024300, C536S024310

Reexamination Certificate

active

07091039

ABSTRACT:
Proteinase inhibitors comprising a Kunitz domain are disclosed. The Kunitz domain comprises a sequence of amino acid residues as shown in SEQ ID NO:3, wherein the sequence is at least 80% identical to residues 6 through 56 of SEQ ID NO:2. Also disclosed are methods for making the proteinase inhibitors, and expression vectors and cultured cells that are useful within the methods. The proteinase inhibitors may be used as components of cell culture media, in protein purification, and in certain therapeutic and diagnostic applications.

REFERENCES:
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patent: 5834244 (1998-11-01), Dennis et al.
patent: 5880256 (1999-03-01), Dennis et al.
patent: 6376648 (2002-04-01), White et al.
patent: 95/18830 (1995-07-01), None
Lederman et al. A single amino acid substitituion in a common african allele of the CD4 molecule ablates binding of the monoclonal antibody, OKT4. Molecular Immunology 28(11): 1171-1181, 1991.
Li et al. Beta-endorphin omission analogs: dissociation of immunoreactivity from other biological activities. Proc. Natl. Acad. Sci. USA 77(6): 3211-3214, Jun. 1980.
Ngo et al. , “The Computational Complexity, protein structure prediction, and the levinthal paradox” in The Protein Folding Problem and Tertiary Structure Prediction, Merz et al., (ed.) 1994.
Newlands and Skuce, GenBank Accession No. AF043121, 1998.
Percy, GenBank Accession No. Z84479, 1998.
INC1680228, LIFESEQ™ Information Results, Incyte Pharmaceuticals, Inc., 1998.
INC1678559, LIFESEQ™ Information Results, Incyte Pharmaceuticals, Inc., 1998.

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