Kunitz domain polypeptide zkun10

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Blood proteins or globulins – e.g. – proteoglycans – platelet...

Reexamination Certificate

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C530S350000, C530S407000, C435S018000

Reexamination Certificate

active

11082148

ABSTRACT:
We claim the proteinase inhibitors comprising a Kunitz domain, wherein the Kunitz domain comprises amino acid residues 57–107 of SEQ ID NO: 2. We also claim a fusion protein comprising said Kunitz domain. In addition, we claim a method of inhibiting protease degradation or protease activity in a composition which contains plasma proteins and proteases. The proteinase inhibitors is used as components of cell culture media in cell culture process, and as inhibitors of protease-mediated degradation of plasma proteins in protein purification.

REFERENCES:
patent: 6544760 (2003-04-01), Holloway
Sinha et al. (1991) Conversion of the Alzheimer's beta-amyloid precursor protein (APP) Kunitz domain into a potent human neutrophil elastase inhibitor. J. Biol. Chem. vol. 266, No. 31,pp. 21011-21013.
INC5272550, LIFESEQ™ Clone Information Results, Incyte Pharmaceuticals, Inc., 1999.
INC2763021, LIFESEQ™ Clone Information Results, Incyte Pharmaceuticals, Inc., 1999.
P15989, Doliana et al., 1990.
AAR81918, Ladner et al., 1996.
AA846371, Strausberg, 1998.
AQ141660, Mahairas et al., 1998.
Mayer et al.,European Journal of Biochemistry 225: 573-580, 1994.
Bonaldo et al., J. Biol. Chem. 264: 20235-20239, 1989.
Ngo et al., “Computational complexity protein structure prediction, and the levinthal paradox,”The Protein Folding Problem and Tertiary Structure Predictionpp. 491-495, Merz, Jr. K. et al. Eds. Birkhauser, Boston, 1994.
Qin et al., “Functional characterization of Kunitz domains in hepatocyte growth factor activator inhibitor type 2,”FEBS Letters 436:111-114, 1998.
Doerks et al., “Protein annotation: detective work for function prediction,”TIG 14:248-250, 1998.

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