Chemistry: molecular biology and microbiology – Virus or bacteriophage – except for viral vector or...
Patent
1996-09-20
1999-06-01
Mosher, Mary E.
Chemistry: molecular biology and microbiology
Virus or bacteriophage, except for viral vector or...
435 9133, 435372, 4742291, C12N 700, A61K 39245
Patent
active
059087730
ABSTRACT:
The invention provides a cell line comprising Kaposi's sarcoma-associated herpesvirus (KSHV). Preferably, the cell line is a body cavity-based lymphoma cell line, and more preferably, the cell line does not harbor Epstein-Barr virus. Two cell lines designated BC-2 and BC-3 are thus provided. These cell lines can be used in a method of propagating KSHV. The method comprises culturing the cell line, wherein the KSHV within the cell line thereby propagates. The BC-3 cell line, in particular, can be used in a method of propagating KSHV in the absence of Epstein-Barr virus. The method comprises culturing the cell line designated BC-3, wherein the KSHV within the cell line thereby propagates. Further provided is a purified viral suspension of KSHV, as well as a composition comprising purified KSHV and a suitable carrier.
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Arvanitakis Leandros
Cesarman Ethel
Knowles Daniel M.
Mesri Enrique
Cornell Research Foundation Inc.
Mosher Mary E.
Salimi Ali R.
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