Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives
Reexamination Certificate
1999-03-30
2001-03-20
Weber, Jon P. (Department: 1051)
Organic compounds -- part of the class 532-570 series
Organic compounds
Carbohydrates or derivatives
C435S100000
Reexamination Certificate
active
06204377
ABSTRACT:
BACKGROUND OF THE INVENTION
1. Field of the Invention
The present invention relates to a novel kojibiose phosphorylase, its preparation and uses, more particularly, to a novel kojibiose phosphorylase which hydrolyzes kojibiose in the presence of an inorganic phosphoric acid and/or its salt (hereinafter abbreviated as “inorganic phosphoric acid” throughout the present specification, if not any inconvenience will arise) to form D-glucose and &bgr;-D-glucose-1-phosphoric acid and/or its salt (hereinafter abbreviated as “&bgr;-D-glucose-1-phosphoric acid” throughout the present specification, if not any inconvenience will arise), and which, in reverse, forms kojibiose and inorganic phosphoric acid from &bgr;-D-glucose-1-phosphoric acid and D-glucose, and to the processes of the kojibiose phosphorylase, saccharide compositions containing glucosyl-transferred saccharides produced by using the kojibiose phosphorylase, and compositions containing the saccharide compositions.
2. Description of the Prior Art
Recently, oligosaccharides such as maltose and trehalose and functions thereof have become to be highlighted, and have been studied on their unique and different processes in view of various aspects. There has been known that phosphorylases such as maltose, trehalose, sucrose, cellobiose, and laminaribiose phosphorylases can be used as methods for producing the above oligosaccharides. Various microorganisms are known as sources of these phosphorylases.
The activities and resources of these phosphorylases are summarized in “
Enzyme Handbook
”, published by Asakura-Shoten Publisher, Tokyo, Japan (1982). However, no phosphorylase capable of forming kojibiose has been known, and the actual supply has been desired.
SUMMARY OF THE INVENTION
The present invention provides a novel enzyme, kojibiose phosphorylase, process thereof, saccharide compositions containing the glucosyl-transferred saccharides prepared by using the enzyme, and uses thereof.
To solve the above object and to obtain an unknown kojibiose phosphorylase, the present inventors widely screened microorganisms which produce such an enzyme. As a result, they found that
Thermoanaerobium brockii
, ATCC 35047, belonging to the genus Thermoanaerobium, produces a novel kojibiose phosphorylase and established the preparation. They also obtained a novel and/or conventionally known glucosyl-transferred saccharides, which can be produced by contacting the enzyme with &bgr;-D-glucose-1-phosphoric acid as a saccharide donor in the presence of saccharides, and a process for compositions containing the above novel and/or conventionally known saccharides. Thus, they accomplished this invention.
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Chaen Hiroto
Kubota Michio
Miyake Toshio
Nishimoto Tomoyuki
Browdy and Neimark
Kabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyujo
Weber Jon P.
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