Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
Patent
1997-01-06
1998-06-16
Horlick, Kenneth R.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing saccharide radical
435 6, 435 15, 435810, C12P 1934
Patent
active
057668906
ABSTRACT:
Methods and kits for the use of a non-ionic detergent to suppress enzyme inhibition in a reaction solution due to the presence of inhibiting ionic detergent. Prior to reaction, the reaction mixture is given an effective amount of a non-ionic detergent, and agitated. The enzyme is then added, and the enzymatic reaction is the allowed to proceed. Also disclosed are preferred embodiments of the present invention, and kits for nucleic acid amplification of a biological sample in the presence of an ionic detergent.
REFERENCES:
patent: 5004684 (1991-04-01), Simpson et al.
patent: 5175094 (1992-12-01), Mallonee
patent: 5358690 (1994-10-01), Guirguis
patent: 5538870 (1996-07-01), Noeth et al.
Loeffelholz et al., J. Clin. Micro. 30(11), 2847-2851 (1992).
Herman, et al. Direct Detection of Chlamydia trachomatis in Urogenital Specimens Using a Rapid, PCR-Based Assay System. Abstract, American Society for Microbiology; Mar., 1991.
Helenius, A. and K. Simons. Solubilization of membranes by detergents. Biochim. Biophys. Acta 415:29-79, Elselvier Scientific Publication Co., Amsterdam (1975).
Ey, P.L. and E. Ferber. Calf thymus alkaline phosphatase II; interactions with detergents. Biochim. Biopys. Acta 480:163-177, Elselvier North-Holland Biomedical Press (1977).
Stralfors, P. Et al. Removal of non-ionic detergent from proteins fractionated by electrofusing. Biochim. Biophys. Acta 533:90-97. Elsevier North-Holland Biomedical Press (1978).
Saito, M. Et al. Action of arthrobacter ureafaciens sialidase on sialglycolipid substrates. J. Biol. Chem. 254(16):7845-54 (1979).
Berge, R. Et al. Variations on the activity of microsomal palmitoyl-CoA hydrolase in mixed micelle solutions of palmitoyl-CoA and non-ionic detergents on the Triton X series. Biochim. Biophys. Acta 666:25-35, Elselvier North-Holland Biomedical Press (1981).
Corti, M. Et al. GMI-Ganglioside-Triton X-100 mixed micelles; changes of micellar properties studied by laser-light scattering and enzymatic methods. Chemistry and Physics of Lipids 28:197-214 (1981).
Wittenburg, C. And E. Tripplett. A detergent-activated tyrosinase from xenopus laevis. J. Biol. Chem. 260(23):12542-12546 (1985).
Tandon, S. And P. Horowitz. Detergent-assisted refolding of guanidinium chloride denatured rhodanese. J. Biol. Chem. 262(10):4486-4491 (1987).
Baselski, V. Et al. Comparison of Roche Chlamydia PCR test to McCoy cell culture in obstetric patients. Dept. of Pathology and Obstet. Gynecology, Univ. Of Tenn., Memphis, TN. Abstract from meeting of the American Society for Microbiology: May 26-30, 1992, Atlanta, GA.
Package insert for brochure of Amplicor's Chlamydia trachomatis test manufactured by Roche-Diagnostic Systems, Nutley, New Jersey, Jun. 1993.
Bachmann, et al., "Improvement of PCR amplified DNA sequencing with the aid of detergents", 18(5):1309, (1990).
Gelfand, "Taq DNA Polymerase", PCR Technology--Principles and Applications for DNA Amplification--Stockton Press, Chpt. 2:17-22, (1990).
Kacian Daniel Louis
McAllister Diane Lisa
Fisher Carlos A.
Gen-Probe Incorporated
Horlick Kenneth R.
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