Kits for screening for blood coagulation disorders

Details Kits for screening for blood coagulation disorders Kits for screening for blood coagulation disorders

2006-10-10

2006-10-10

Gitomer, Ralph (Department: 1655)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving blood clotting factor

C435S975000

Reexamination Certificate

active

07118880

ABSTRACT:
In vitro methods for qualitative screening and/or quantitative determination of the functional activity of components of the Protein C anticoagulant pathway of blood coagulation are described. The methods entail measuring the conversion rate of a substrate by an enzyme, the activity of which is related to the Protein C anticoagulant activity, in a blood sample of a human comprising coagulation factors and said substrate, after at least partial activation of coagulation through the intrinsic, extrinsic or common pathway and triggering coagulation by adding calcium ions; and comparing said conversion rate with the conversion rate of a normal human blood sample determined in the same way. The methods include the addition of additional metal ions to the sample to enhance activity, sensitivity and resolution. Kits and reagents for use in the methods are also provided.

REFERENCES:
patent: 5001069 (1991-03-01), Bartl et al.
patent: 5055412 (1991-10-01), Proksch
patent: 5308756 (1994-05-01), van de Waart et al.
patent: 5439802 (1995-08-01), Rosén
patent: 5552296 (1996-09-01), Adema et al.
patent: 5637452 (1997-06-01), Speck
patent: 5637492 (1997-06-01), Dawson et al.
patent: 6083905 (2000-07-01), Voorberg et al.
patent: 6245573 (2001-06-01), Spillert
patent: 6395501 (2002-05-01), Rosen et al.
patent: 6426192 (2002-07-01), Stocker et al.
patent: 6730490 (2004-05-01), Rosen et al.
patent: 6800450 (2004-10-01), Rosen et al.
patent: 6838251 (2005-01-01), Exner
patent: 2002/0019021 (2002-02-01), Kraus
patent: 2002/0115127 (2002-08-01), Rosén et al.
patent: 2003/0044871 (2003-03-01), Cutsforth et al.
patent: 2003/0199014 (2003-10-01), Rosen et al.
patent: 2003/0207343 (2003-11-01), Kraus
patent: 2004/0053351 (2004-03-01), Fischer et al.
patent: 2005/0059132 (2005-03-01), Dahlback et al.
patent: 0 203 509 (1986-05-01), None
patent: 0 567 636 (1997-01-01), None
patent: WO 93/10262 (1993-05-01), None
patent: WO 99/41615 (1999-08-01), None
Bernado et al.: Surface-Independent Acceleration of Factor XII Activation by Zinc Ions,J. Biol. Chem.268(17):12468-12476 (1993).
Butenas et al.: Cooperative Interaction of Divalent Metal Ions, Substrate, and Tissue Factor with Factor VIIa,J. Biol. Chem.33:3449-3456 (1994).
Gable et al.: Protac, A Commercially Available Protein C Activator From the Venom ofAgkistrodon Contortrix Contortrix, Can Activate Factor V and Factor VIII,Thrombosis Research86(1):79-84 (1997).
Heeb et al.: Identification of Divalent Metal Ion-Dependent Inhibition of Activated Protein C by Alpha 2-Macroglobulin and Alpha 2-Antiplasmin in Blood and Comparsions to Inhibition of Factor Xa, Thrombin, and Plasmin,J. Biol. Chem266(26):17606-17612 (1991).
Liebman et al.: The Factor IX Phospholipid-binding Site is Required for Calcuim-Dependent Activation of Factor IX by Factor Xia,J. Biol. Chem.262(16):7605-7612 (1987).
Pedersen et al.: Inhibition of Recombinant Human Blood Coagulation Factor VIIa Amidolytic and Proteolytic Activity by Zinc Ions,Thrombosis and Haemostasis65(5):528-534 (1991).
Sekiya et al.: Regulation of the Tertiary Structure and Function of Coagulation Factor IX by Magnesium (II) Ions*,J. Biol. Chem.270(24):14325-14331 (1995).
Shore et al.: Acceleration of Surface-Dependent Autocatalytic Activation of Blood Coagulation Factor XII by Divalent Metal Ions,J. Biol. Chem.26: 2250-2258 (1987).
Patent Cooperation Treaty, International Search Report, International Application No. PCT/EP99/01599, mailed on Jul. 27, 1999, 8 pages.
Amphlett et al., (1978), “The Binding of Metal Ions to Bovine Factor IX,”J. Biol. Chem., 253(19):6774-6779.
Bajaj et al., (1977), “Interaction of Manganese with Bovine Factor X,”J. Biol. Chem., 252(14)4758-4761.
Byrne et al., (1980), “Metal Ion Specificity of the Conversion of Bovine Factors IX, Ixα, and Ixαα to Bovine Factor Ixαβ,”J. Biol. Chem., 2554)1430-1435.
Morita et al., (1997), “A New Cascade Theory of Blood Coagulation: Magnesium (II) is a Crucial Constituent of the Blood Coagulation Cascade,”Thromb. Haemost., 78(Supp):430-Abstract PS-1755.
Persson et al., (1995), “Structurally and Functionally Distinct Ca2+ Binding Sites in the γ-Carboxyglutamic Acid-containing Domain of Factor VIIa,”Eur. J. Biochem., 234:293-300.
Prendergast et al., (1977), “Differentiation of Metal Ion-Induced Transitions of Prothrombin Fragment 1,”J. Biol. Chem., 252(3)840-850.
Sekiya et al., (1996), “Magnesium(II) Is a Crucial Constituent of the Blood Coagulation Cascade,”J. Biol. Chem., 271(15):8541-8544.

Affiliated with

Also associated with

Rating

Kits for screening for blood coagulation disorders does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Kits for screening for blood coagulation disorders, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Kits for screening for blood coagulation disorders will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-3710964