Kits for measuring cellular transmembrane potential changes

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism

Reexamination Certificate

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

C435S975000, C544S242000

Reexamination Certificate

active

11020536

ABSTRACT:
The invention encompasses kits for an improved method for measuring membrane potential using compounds of the formula I as potentiometric probes. These probes may be used in combination with other fluorescent indicators such as Indo-1, Fura-2, and Fluo-3, such probes may be used in microplate reading devices such as FLIPR™, fluorescent imaging plate reader, sold by Molecular Devices Corp., of Sunnyvale, Calif.; flow cytometers; and fluorometers. Such probes are used to measure membrane potential in live cellswherein X is O or S; andn is 1 or 2.

REFERENCES:
patent: 4370401 (1983-01-01), Winslow et al.
patent: 6287758 (2001-09-01), Okun et al.
patent: 6455271 (2002-09-01), Little, II et al.
patent: 6537771 (2003-03-01), Farinas et al.
patent: 6596522 (2003-07-01), Tsien et al.
patent: 6852504 (2005-02-01), Klaubert et al.
patent: 1231884 (1971-05-01), None
patent: 2136590 (1971-05-01), None
patent: 1231884 (1984-09-01), None
patent: WO 01/42211 (2001-06-01), None
patent: WO 01/42211 (2001-06-01), None
patent: WO 03/014701 (2003-02-01), None
Sabry S. et al. Sensitive Spectrofluorimetric and Spectrophotometric . . . J of Pharmaceutical and Biomedical Analysis 22(2)257-264, 2000.
Nakashima K. Development and Application of Organic Reagents . . . Chem Pharm Bull 33(12)5380-4, 1985.
Synthese und Reaktionen Quartarer N-[β-Formyl-Vinyl]-Ammuniumsalze, Fisher,Chem. Ber., vol. 102, pp. 2609-2620, 1969.
Untersuchungen zur Reaktivitat des 5,6-Dihydro-2-methyl-5-oxo-4H-1,4-oxazin-3-carbonsaureethylesters, Bartsch et al.,Arch. Pharm., vol. 315, pp. 761-766, 1982.
Optical Measurement of Membrane Potential in Cells, Organelles, and Vesicles, Freedman et al.,Methods in Enzymology, vol. 172, pp. 102-122, 1989.
Fluorescent Indicators of Membrane Potential: Microspectrofluorometry and Imaging, Gross et al.,Methods in Cell Biology, vol. 30, pp. 193-218, 1989.
Potential-Sensitive Molecular Probes in Membranes of Bioenergetic Relevance, Smith,Biochimica et Biophysico Acta, vol. 1016, pp. 1-28, 1990.
The Use of the Potential-Sensitive Fluorescent Probe Bisoxonol in Mast Cells, Bronner et al.,Biochimica et Biophysica Acta, vol. 1070, pp. 321-331, 1991.
Peroxyoxalate Chemiluminescence Detection of Condensates of Malondialdehyde With Thiobarbituric Acids Using a Flow System, Biomedical Chromatography, vol. 6, pp. 55-58, 1992.
Fast Multisite Optical Measurement of Membrane Potential, Wu et al.,Fluorescent and Luminescent Probes for Biological Activity, pp. 389-404, 1993.
Confocal Microscopy of Potentiometric Fluorescent Dyes, Loew,Methods in Cell Biology, vol. 38, pp. 195-209, 1993.
Characterization of Potentiometric Membrane Dyes, Loew,Adv. Chem. Ser., vol. 235, pp. 151-173, 1994.
Cell Membrane Potential Analysis, Shapiro,Methods in Cell Biology, vol. 41, pp. 121-133, 1994.
Slow Fluorescent Indicators of Membrane Potential: A Survey of Different Approaches to Probe Response and Analysis, Plasek,Journal of Photochemistry and Photobiology, vol. 33, pp. 101-124, 1996.
Improved Indicators of Cell Membrane Potential That Use Fluorescence Resonance Energy Transfer, Gonzalez,Chem. Biol., vol. 4, pp. 269-277, 1997.
Detection of Cell Viability in Cultures of Hyperthermophiles, Beck et al.,FEMS Microbiology Letters, vol. 47, pp. 11-14, 1997.
Assessment of the Effect of Amphotericin B on the Vitality of Candida Albicans, Liao et al.,Antimicrobial Agents and Chemotherapy, vol. 43, No. 5, pp. 1034-1041, May 1999.
Antifungal Susceptibility Testing of Candida Species by Flow Cytometry, Lee et al.,J. Korean Med. Science, vol. 14, pp. 21-26, 1999.
Fluorescent and Luminescent Probes for Biological Activity, Loew, 2nd Ed., pp. 210-221, 1999.
Concepts in Imaging and Microscopy: Imaging Membrane Potential With Voltage-Sensitive Dyes, Zochowski et al.,Biol. Bull., vol. 198, pp. 1-21, Feb. 2000.
Sensitive Spectrofluorimetric and Spectrophotometric Methods for the Determination of Thonzylamine Hydrochloride in Pharmaceutical Preparations Based on Coupling with Dimethylbarbituric Acid in Presence of Dicyclohexylcarbodiimide, Sabry et al.,Journal of Pharmaceutical and Biomedical Analysis, vol. 22, pp. 257-264, 2000.
Convenient Fluorescence-Based Methods to Measure Membrane Potential and Intracellular pH in the Archaeon Methanobacterium Thermoautotrophicum, de Poorter et al.,Journal of Microbiological Methods, vol. 47, pp. 233-241, 2001.
International Search Report for PCT/US02/25046 (DATE).
Baxter, Deborah F. et al. (Jan. 2001).A Novel Voltage-Sensitive Fluorescent Dye for Ion Channel and Transporter Assays, Biophysical Journal, vol. 80, No. 1, Part 2, p. 143a (abstract).

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Kits for measuring cellular transmembrane potential changes does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Kits for measuring cellular transmembrane potential changes, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Kits for measuring cellular transmembrane potential changes will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-3927425

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.