Kits for high temperature reverse transcription of RNA

Details Kits for high temperature reverse transcription of RNA Kits for high temperature reverse transcription of RNA

1994-09-22

1997-06-24

Zitomer, Stephanie W.

Chemistry: natural resins or derivatives; peptides or proteins;

Proteins, i.e., more than 100 amino acid residues

435 6, 536 2433, C07K 14195, C12Q 168, C07H 2100

Patent

active

056418647

ABSTRACT:
Methods are provided for the replication and amplification of RNA sequences by thermoactive DNA polymerases. In a preferred embodiment, high temperature reverse transcription is coupled to nucleic acid amplification in a one tube, one enzyme procedure using a thermostable DNA polymerase. Methods for eliminating carry over contamination of amplifications due to prior reverse transcription reactions are also provided.

REFERENCES:
patent: 4683195 (1987-07-01), Mullis et al.
patent: 4683202 (1987-07-01), Mullis
patent: 4889818 (1989-12-01), Gelfand et al.
patent: 5035996 (1991-07-01), Hartley
patent: 5320958 (1994-06-01), Inouye et al.
1988 Stratagene Catalog, p. 39.
Kallen et al., 1961, "The Occurrence of a New Pyrimidine Base Replacing Thymine in a Bacteriophage DNA: 5-Hydroxymethyl Uracil" J. Mol. Biol. 5:248-250.
Karkas et al., Feb., 1972, "Action of DNA Polymerase I of Escherichia coli with DNA-RNA Hybrids as Templates" Proc. Natl. Acad. Sci. USA 69(2):398-402.
Leob et al., Mar., 1973, "Copying Natural RNAs with E. coli DNA Polymerase I" Nature New Biology 242:66-69.
Karkas, Dec., 1973, "Reverse Transcription by Escherica coli DNA Polymerase I" Proc. Natl. Acad. Sci. USA 70(12)Part II:3834-3838.
Brown, 1974, "Chemical Reactions of Polynucleotides and Nucleic Acids" Basic Principles in Nucleic Acid Chemistry 2:41-45.
Gulati et al., Apr., 1974, "Conditions for Using DNA Polymerase I as an RNA-Dependent DNA Polymerase" Proc. Natl. Acad. Sci. USA 71(4):1035-1039.
Temin and Mizutani, The Enzymes, Chapter 7, Entitled "RNA Tumor Virus DNA Polymerases," Paul Boyer, Acedemic Press, Inc., London, 1974 ed., pp. 211-235.
Travaglini and Leob, 1974, "Ribonucleic Acid Dependent Deoxyribonucleic Acid Synthesis by Escherichia coli Deoxyribonucleic Acid Polymerase I. Characterization of the Polymerization Reaction" Biochemistry 13(15):3010-3017.
Chien et al., 1976, "Deoxyribonucleic Acid Polymerase from the Extreme Thermophile Thermus aquaticus" J. Bacteriology 127(3):1550-1557.
Houdebine, 1976, "Synthesis of DNA Complementary to the mRNAs for milk proteins by E. coli DNA Polymerase I" Nucleic Acids Research 3(3):615-630.
Mizutani and Temin, 1976, "Incorporation of Noncomplementary Nucleotides at High Frequencies by Ribodeoxyvirus DNA Polymerases and Escherichia coli DNA Polymerase I" Biochemistry 15:1510-1516.
Travaglini et al., 1976, "Template Recognition and Chain Elongation in DNA Synthesis in Vitro" J. Mol. Biol. 106:605-621.
Kaledin et al., 1980, "Isolation and Properties of DNA Polymerase From Extremely Thermophilic Bacterium Thermus aquaticus YT1" Biokhimiya 45(4):644-651.
"Metal Ions in Genetic Information Transfer" in Advances in Inorganic Biochemistry vol. 3, pp. 26-29, 32-35, and 42-46 published by Elsevier North Holland Inc., New York, New York, Eds. Eichorn, Gunther, Louis and Marzill, Luigi, 1981.
Ruttimann et al., 1985, "DNA Polymerases from the Extremely Thermophilic Bacterium Thermus thermophilus HB-8" Eur. J. Biochem. 149:41-46.
Jones and Foulkes, 1989, "Reverse Transcription of mRNA by Thermus aquaticus DNA Polymerase" Nucleic Acids Research 17(20):8387-8388.
Kawasaki, 1989, "Amplification of RNA Sequences via Complementary DNA (cDNA)" Amplifications 3:4-6.
Lawyer et al., Apr. 1989, "Isolation, Characterization, and Expression in Escherichia coli of the DNA Polymerase Gene from Thermus aquaticus" Biological Chemistry 264(11):6427-6437.
Shimomaye and Salvato, 1989, "Use of Avian Myeloblastosis Virus Reverse Transcriptase at High Temperature for Sequence Analysis of Highly Structured RNA" Gene Anal. Techn. 6:25-28.
Tabor and Richardson, 1989, "Effect of Manganese Ions on the Incorporations of Dideoxynucleotides by Bacteriophage T7 DNA Polymerse and Escherichia coli DNA Polymerase I" Proc. Natl. Acad. USA 86:4076-4080.
Mocharla et al., 1990, "Coupled Reverse Transcription-Polymerase Chain Reaction (RT-PCR) as a Sensitive and Rapid Method for Isozyme Genotyping" Gene 93:271-275.
Shaffer et al., 1990, "Amplications, Detection, and Automated Sequencing of Gibbon Interleukin-2 mRNA by Thermus aquaticus DNA Polymerase Reverse and Polymerase Chain Reaction" Analytical Biochemistry 190):292-296.
Tse and Forget, 1990, "Reverse Transcription and Direct Amplification of Cellular RNA Transcripts by Taq Polymerase" Gene 88:293-296.
Levy and Teebor, 1991, "Site Directed Substitution of 5-Hydroxymethyluracil for Thymine in Replicating Phi-X-174am3 DNA via Synthesis of 5-Hydroxymethyl-2'-Deoxyuridine-5'-Triphosphate" Nucleic Acids Research 19(12):3337-3343.
Lou et al., 1991, "Renin Gene Expression in Various Tissues Determined by Single-Step Polymerase Chain Reaction" Clinical and Experimental Pharmacology and Physiology 18:357-362.

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