Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
Patent
1995-06-07
1998-01-06
Marschel, Ardin H.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing saccharide radical
435 6, 435 912, 536 221, 536 231, 536 243, 536 2433, 536 2532, C12Q 168, C12P 1934, C07H 1900, C07H 2102
Patent
active
057053650
ABSTRACT:
Methods and kits for relating initial amounts of target nucleic acids present in a sample to target-specific amplification products. It has been discovered that the transcription-mediated amplification system is capable of producing a quantitative relationship between target input and target-specific output. Further, the the present invention relates to methods for carefully controlling this relationship resulting in an unexpectedly high degree of reproducability. Also described are useful methods for extending the dynamic range of transcription-based amplification systems.
REFERENCES:
patent: 4683195 (1987-07-01), Mullis et al.
patent: 4786600 (1988-11-01), Kramer et al.
patent: 4946958 (1990-08-01), Campbell et al.
patent: 5030557 (1991-07-01), Hogan
patent: 5130238 (1992-07-01), Malek et al.
patent: 5283174 (1994-02-01), Arnold et al.
patent: 5399491 (1995-03-01), Kacian et al.
patent: 5476774 (1995-12-01), Wang et al.
Van Gemen, B., "The One Tube Quantitative HIV-1 RNA NASBA. Precision Accuracy and Application", PCR Methods and Applications, vol. 4, 4:177-184, Feb. 1995.
Arnold, et al., "PCR-based Quantitation Of Low Levels of HIV-1 DNA By Using An External Standard", Genetic Analysis Techniques and Applications, vol. 9 4:113-116, Aug. 1992.
Persing, D. In vitro Amplification Techniques. Diagnostic Medical Microbiology: Principles and Applications, Chp.3 at p. 65, (American Society for Microbiology; 1993 ed.).
Chelly, J., et al. Transcription of the dystrophin gene in human muscle and non-muscle tissues. 333 Nature 858-860 (1988).
Van Geme et al. A one-tube quantitative HIV-1 RNA NASBA nucleic acid amplification assay using electrochemiluminescent (ECL) labelled probes. 49 J. Vir. Meth. 157-168 (1994).
Modak & Marcus. Specific Inhibition of DNA Polymerase-Associated RNase H by DNA. 22 J. Virol. 243-246 (1977).
Marcus, et al. Reverse Transcriptase-Associated RNase H Activity. 27 J. Virol. 576-581 (1978).
Okayama, et al. High-Efficiency Cloning of Full-Length cDNA. 154 Methods Enzymol. 3-28 (1987).
Desrosiers, R. Virus Purification, Preparation of Infectious Virus Stock, and Virus Storage. Techniques in HIV Research, pp. 121-127, (Stockton Press, New York 1990).
Billyard Elizabeth R.
Castillo Maria R.
Gonzales Frank R.
Harvey Richard C.
Kacian Daniel Louis
Fisher Carlos A.
Gen-Probe Incorporated
Marschel Ardin H.
Riley Jezia
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