Kits for detecting amplification of human MDM2

Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives

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206569, C07H 2102, C07H 2104, B65D 6900

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056060440

ABSTRACT:
A human gene has been discovered which is genetically altered in human tumor cells. The genetic alteration is gene amplification and leads to a corresponding increase in gene products. Detecting that the gene, designated hMDM2, has become amplified or detecting increased expression of gene products is diagnostic of tumorigenesis. Human MDM2 protein binds to human p53 and allows the cell to escape from p53-regulated growth.

REFERENCES:
Fakharzadeh, et al., "Tumorigenic Potential Associated with Enhanced Expression of a GEne That is Amplified in a Mouse Tumor Cell Line", The EMBO Journal, 10(6):1565-1569 (1991).
Hinds, et al., "Mutant p53 DNA Clones From Human Colon Carcinomas Cooperate With Ras in Transforming Primary Rat Cells: A Comparison of the Hot Spot Mutant Phenotypes", Cell Growth & Differentiation, 1:561-580 (1990).
Romkes, et al., "Cloning and Expression of cDNA for Multiple Members of the Human Cytochrome P450IIC Subfamily", Biochemstry, 30(13):3247-3255 (1991).
Momand, et al., "The MDM2 Oncogene Product Forms a Complex with the p53 Protein and Inhibits 53-Mediated Transactivation," Cell:69:1237-1245 (1992).
Oliner, et al., "Amplification of a Gene Encoding a p53-Associated Protein in Human Sarcomas", Nature, 358:80-83 (1992).
Ladanyi, et al., "MDM2 Gene Amplification in Metastatic Osteosarcoma", Cancer Research, 53:16-18 (1993).
Leach, et al., "p53 Mutation and MDMS Amplification in Human Soft Tissue Sarcomas", Cancer Research 53:2231-2234 (1993).
Oliner, et al., "Oncoprotein MDM2 Conceals the Activation Domain of Tumour Suppressor p53", Nature, 362(6423):857-860 (1993).
Cahilly, et al., "Molecular Analysis and Chromosomal Mapping of Amplified Genes Isolated from a Transformed Mouse 3T3 Cell Line", Somatic Cell and Molecular GEnetics, 13(3):235-244 (1987).
Bueso-Ramos, et al., "The Human MDM-2 Oncogene is Overexpressed in Leukemias", Blood, 82(9):2617-2623 (1993).

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