Kits for detecting a target nucleic acid with blocking oligonucl

Details Kits for detecting a target nucleic acid with blocking oligonucl Kits for detecting a target nucleic acid with blocking oligonucl

1995-05-04

1998-01-27

Horlick, Kenneth R.

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

435 6, 435 912, 536 231, 536 243, C07H 2104, C12P 1934

Patent

active

057123861

ABSTRACT:
A method for detecting a target nucleic acid, which method includes the steps of (i) amplifying the target nucleic acid to obtain an amplification product using a polymerase, a first primer with or without a segment noncontiguous to a first priming sequence, and a second primer with or without a segment noncontiguous to a second priming sequence in the presence of an oligonucleotide which is incapable of acting as a primer for the polymerase, wherein the oligonucleotide has at least 5 consecutive nucleotides fully complementary to at least 5 consecutive nucleotides of the first primer; and (ii) detecting the presence of the target nucleic acid by monitoring the amplification thereof.

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Blanco, L. et al. Highly Officient DNA Synthesis by the Phage .o slashed.29 DNA Polymerase (J. Biol. Chem. (1989) 254:8935-8940.
P. Holland et al., "Detection of specific polymerase chain reaction product . . . ", Proc. Natl. Acad. Sci. USA 88:7276-7280 (1991).
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Morrison, L. E., Halder, T.C. and Slols, L.M., "Solution-Phase Detection of Polynucleotides Using Interacting Fluorescent Labels and Competitive Hybridization", Analytical Biochemistry 183:231-244 (1989).

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