Kits for amplifying and detecting nucleic acid sequences, includ

Details Kits for amplifying and detecting nucleic acid sequences, includ Kits for amplifying and detecting nucleic acid sequences, includ

1994-09-27

2000-03-21

Martinell, James

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Transferase other than ribonuclease

435 6, 435 912, 536 231, C12N 912

Patent

active

060401664

ABSTRACT:
The present invention is directed to a process for amplifying any target nucleic acid sequence contained in a nucleic acid or mixture thereof using a thermostable enzyme. The process comprises treating separate complementary strands of the nucleic acid with a molar excess of two oligonucleotide primers, extending the primers with a thermostable enzyme to form complementary primer extension products which act as templates for synthesizing the desired nucleic acid sequence, and detecting the sequence so amplified. The steps of the reaction can be repeated as often as desired and involve temperature cycling to effect hybridization, promotion of activity of the enzyme, and denaturation of the hybrids formed.

REFERENCES:
patent: 4358535 (1982-11-01), Falkow et al.
patent: 4486539 (1984-12-01), Ranki et al.
patent: 4683195 (1987-07-01), Mullis et al.
patent: 4683202 (1987-07-01), Mullis
patent: 4851331 (1989-07-01), Vary et al.
patent: 4889818 (1989-12-01), Gelfand et al.
Kleppe et al., 1971, "Studies on Polynucleotides--Repair Replication of Short Synthetic DNA's as Catalyzed by DNA Polymerases" J. Mol. Biol. 56:341-361.
Besmer et al., 1972, "Studies on Polynucleotides--Hybridization of Polydeoxynucleotides with Tyrosine Transfer RNA Sequences to the r-Strand of phi-80 psu+III DNA" J. Mol. Biol. 72:502-522.
Khorana et al., 1972, "Studies on Polynucleotides--Total Synthesis of the Structural Gene for an Alanine Transfer Ribonucleic Acid from Yeast" J. Mol. Biol. 72:209-217.
Panet and Khorana, 1974, "The Linkage of Deoxyribopolynucleotide Templates to Cellulose and its Use in Their Replication" J. Biological Chemistry 249(16):5213-5221.
Olson et al., Jan., 1975, "Enzymatic Multiplication of a Chemically Synthesized DNA Fragment" Nucleic Acids Research 2(1):43-60.
Research Awards Index, Fiscal Year 1976, p. 2565, Publication No. (NIH) 77-200.
Research Awards Index, Fiscal Year 1977, p. 2812, Publication No. (NIH) 78-200.
Research Awards Index, Fiscal Yeae 1978, page number unavailable, Publication No. (NIH) 79-200.
Salser, in Genetic Engineering, "Cloning cDNA Sequences: A General Technique for Propagating Eurakyotic Gene Sequences in Bacterial Cells" 1978, Chakrabarty (ed.), CRC Press Inc., Boca Raton Florida, pp. 53-81.
Research Awards Index, Fiscal Year 1979, p. 1742, Publication No. (NIH) is unavailable.
Houghton et al., 1980, "The Complete Amino Acid Sequence of Human Fibroblast Interferon as Deduced Using Synthetic Oligodeoxyribonucleotide Primers of Reverse Transcriptase" Nucleic Acids Research 8(13):2885-2894.
Research Awards Index, Fiscal Year 1980, pp. 1312 and 1802, Publication No. (NIH) 81-200.
Hong, 1981, "A Method for Sequencing Single-Stranded Cloned DNA in Both Directions" Bioscience Reports 1:243-252.
Kaboev et al., Jan., 1981, "Purification and Properties of Deoxyribonucleotide Acids Polymerase from Bacillus Stearothermophilus" J. Bacteriology 145(1):21-26.
Kaledin et al., Sep., 1981, "Isolation and Properties of DNA Polymerase From the Extremely Thermophilic Bacterium Thermus flavus" in Biochemistry, a Translation of Biokhimrya 46(9):1576-1584.
Wallace et al., 1981, "A set of Synthetic Oligodeoxyribonucleotide Primers for DNA Sequencing in the Plasmid Vector pBR322" Gene 16:21-26.
Winter et al., 1981, "The Use of Synthetic Oligodeoxynucleotide Primers in Cloning and Sequencing Segment 8 of Influenza Virus (A/PR/8/34)" Nucleic Acids Research 9(2):237-245.
Research Awards Index, Fiscal Year 1981, p. 1666, Publication No. (NIH) 82-200.
Kaledin et al., Nov., 1982, "Isolation and Properties of DNA Polymerase From the Extremely Thermophilic Bacterium Thermus ruber" in Biochemistry, a Translation of Biokhimiya 47(11):1785-1791.
Okayama and Berg, Feb., 1982, "High-Efficiency Cloning of Full-Length cDNA" Molecular and Cellular Biology 2(2):161-170.
Hong, 1982, "Sequencing of Large Double-Stranded DNA Using the Dideoxy Sequencing Technique" Bioscience Reports 2:907-912.
Rossi et al., Aug., 1982, "An Alternate Method for Sythesis of Double-Stranded DNA Segments" J. Biological Chemistry 257(16):9226-9229.
Conner et al., Jan., 1983, "Detection of Sickle Cell Beta-S Globin Allele by Hybridization With Synthetic Oligonucleotides" Proc. Natl. Acad. Sci. 80:278-282.
Das et al., Mar., 1983, "Use of Synthetic Oligonucleotide Probes Complementary to Genes for Human HLA-DR alpha and beta as Extension Primers for the Isolation of 5'-Specific Genome Clones" Proc. Natl. Acad. Sci. 80:1531-1535.
Deininger, 1983, "Approaches to Rapid DNA Sequence Analysis" Analytical Biochemistry 135:247-263.
Molecular Biological's catalog from Pharmacia, 1984, p. 49.
Schold et al., 1984, "Laboratory Methods Oligonucleotide-Directed Mutagenesis Using Plasmid DNA Templates and Two Primers" DNA 3(6):469-477.
Zoller et al., 1984, "Oligonucleotide-Directed Mutagenesis: A Simple Method Using Two Oligonucleotide Primers and a Single-Stranded DNA Template" DNA 3(6):479-488.
Chen and Seeburg, 1985, "Supercoil Sequencing: A Fast and Simple Method for Sequencing Plasmid DNA" DNA 4(2):165-170.
Ruttiman et al., 1985, "DNA Polymerases From the Extremely Thermophilic Bacterium Thermus Thermophilus HB-8" Eur. J. Biochem. 149:41-46.
Zagursky et al., 1985, "Rapid and Easy Sequencing of Large Linear Double-Stranded DNA and Supercoiled Plasmid DNA" Gene Anal Techn. 2:89-94.
Templeton, 1992, "The Polymerase Chain Reaction--History, Methods, and Applications" Diagnostic Molecular Pathology 1(1):58-72.
Khorana Research Proposal, National Science Foundation (1972) (Trial Exhibit A-17).
Putative Kleppe Lecture from 1969 Gordon Conference.
Boehringer Mannheim Product Catalogue, Jan., 1985, pp. IV, 15 and 17.
NCI Grant Application CA No. 11981-01 for Apr. 1, 1970, to Mar. 31, 1975, submitted by Dr. Khorana.
Goeddel et al., 1980, "Synthesis of Human Fibroblast Interferon by E. coli" Nucleic Acids Research 8(18):4057-4073.
Houghton et al., 1990, "The Amino-Terminal Sequence of Human Fibroblast Interferon as Deduced From Reverse Transcripts Obtained Using Synthetic Oligonucleotide Primers" Nucleic Acids Research 8(9):1913-1931.
Derynck et al., Aug., 1984, "Human Transforming Growth Factor--Alpha Precurson Structure and Expression in E. coli" Cell 38:287-297.
Itakura et al., 1984, "Synthesis and Use of Synthetic Oligonucleotides" Ann. Rev. Biochem. 53:323-356.
Wood et al., 1984, "Expression of Active Human Factor VIII From Recombinant DNA Clones" Nature 312:330-337.
Aug., 1985, Promega Notes 2:1-8.
Saiki et al., Dec., 1985, "Enzymatic Amplification of Beta-Globin Genomic Sequences and Restriction Site Analysis for Diagnosis of Sickle Cell Anemia" Science 23:1350-1354.
Southern, 1975, "Detection of Specific Sequences Among DNA Fragments Separated by Gel Electrophoresis" J. Mol. Biol. 98:503-517.
Britten and Kohne, Aug., 1968, "Repeated Sequences in DNA" Science 161(3841):529-540.
Wetmur and Davidson, 1968, "Kinetics of Renaturation of DNA" J. Mol. Biol. 31:349-370.
Kochetkov and Budovskii, Oganic Chemistry of Nucleic Acids, London and New York, Plenum Press, 1971, Chapter 4, Entitled "The Secondary Structure of Nucleic Acids" pp. 224-226.
Summers et al., Nov., 1975, "Genome of Hepatitis B Virus: Restriction Enzyme Cleavage and Structure of DNA Extracted from Dane Particles" Proc. Natl. Acad. Sci. USA 72(11):4597-4601.
Chien et al., Sep., 1976, "Deoxyribonucleic Acid Polymerase From the Extreme Thermophile Thermus aquaticus" J. Bacteriology 127(3):1550-1557.
Gait and Sheppard, Dec., 1976, "A Polyamide Support for Oligonucleotide Synthesis" J. American Chemical Society 98(26):8514-8516.
Sanger et al., Dec., 1977, "DNA Sequencing with Chain-Terminating Inhibitors" Proc. Natl. Acad. Sci. USA 74(12):5463-5467.
Kuhn et al., 1978, "DNA Helicases" CSH-Quantitative Biology 43:63-67.
Lehninger, Biochemistry, 2nd ed., New York, NY, Worth Publishers, Inc., 1979, Chapter 32, entitled "Replication and Transcription DNA" pp. 897-899, 904, and 905.
Caton and Robertson, 1979, "New Procedure for the Production of Influenza Virus-Specific Double-Stranded DNA's " Nucleic Acids Research 7(6):1445-1455.
Alvarado-Urbina et al., 1979, "Automated Synthesis of Ge

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