Kits employing generalized target-binding e-tag probes

Details Kits employing generalized target-binding e-tag probes Kits employing generalized target-binding e-tag probes

2006-02-21

2006-02-21

Siew, Jeffrey (Department: 1637)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C435S808000, C536S023100, C536S024300, C536S025320, C204S450000, C206S569000

Reexamination Certificate

active

07001725

ABSTRACT:
Kits for the multiplexed detection of the binding of, or interaction between, one or more ligands and target antiligands are provided. Detection involves the release of identifying tags as a consequence of target recognition. The kits include sets of electrophoretic tag probes or e-tag probes, a capture agent and optionally a cleaving agent. The e-tag probes comprise a detection region and a mobility-defining region called the mobility modifier, both linked to a target-binding moiety. In using the kits, target antiligands are contacted with a set of e-tag probes and the contacted antiligands are treated with a selected cleaving agent resulting in a mixture of e-tag reporters and uncleaved and/or partially cleaved e-tag probes. The mixture is exposed to a capture agent effective to bind to uncleaved or partially cleaved e-tag probes, followed by electrophoretic separation. In a multiplexed assay, different released e-tag reporters may be separated and detected providing for target identification.

REFERENCES:
patent: 4331590 (1982-05-01), Bocuslaski
patent: 4650750 (1987-03-01), Giese
patent: 4675300 (1987-06-01), Zare et al.
patent: 4709016 (1987-11-01), Giese
patent: 4780421 (1988-10-01), Kameda
patent: 5324401 (1994-06-01), Yeung et al.
patent: 5340716 (1994-08-01), Ullman
patent: 5360819 (1994-11-01), Giese
patent: 5470705 (1995-11-01), Grossman et al.
patent: 5516636 (1996-05-01), McCapra
patent: 5516931 (1996-05-01), Giese
patent: 5536834 (1996-07-01), Singh et al.
patent: 5560811 (1996-10-01), Briggs et al.
patent: 5565324 (1996-10-01), Still et al.
patent: 5573906 (1996-11-01), Bannwarth et al.
patent: 5580732 (1996-12-01), Grossman et al.
patent: 5602273 (1997-02-01), Giese
patent: 5604104 (1997-02-01), Giese
patent: 5610020 (1997-03-01), Giese
patent: 5622929 (1997-04-01), Willner et al.
patent: 5624800 (1997-04-01), Grossman et al.
patent: 5650270 (1997-07-01), Giese
patent: 5703222 (1997-12-01), Grossman et al.
patent: 5709994 (1998-01-01), Pease
patent: 5719028 (1998-02-01), Dahlberg et al.
patent: 5721099 (1998-02-01), Still et al.
patent: 5723591 (1998-03-01), Livak et al.
patent: 5756726 (1998-05-01), Hemmi et al.
patent: 5789172 (1998-08-01), Still et al.
patent: 5807675 (1998-09-01), Davalian
patent: 5807682 (1998-09-01), Grossman et al.
patent: 5811239 (1998-09-01), Frayne
patent: 5843655 (1998-12-01), McCall
patent: 5843666 (1998-12-01), Akhavan-Tafti et al.
patent: 5846839 (1998-12-01), Gallop
patent: 5851770 (1998-12-01), Babon et al.
patent: 5874213 (1999-02-01), Cummins et al.
patent: 5876930 (1999-03-01), Livak et al.
patent: 5989871 (1999-11-01), Grossman et al.
patent: 5998140 (1999-12-01), Dervan et al.
patent: 6001579 (1999-12-01), Still et al.
patent: 6027890 (2000-02-01), Ness
patent: 6045676 (2000-04-01), Mathies et al.
patent: 6090947 (2000-07-01), Dervan et al.
patent: 6251581 (2001-06-01), Ullman
patent: 6312893 (2001-11-01), Van Ness
patent: 6331530 (2001-12-01), Breslow et al.
patent: 6368874 (2002-04-01), Gallop
patent: WO 93/06121 (1993-04-01), None
patent: WO 96/24061 (1996-08-01), None
patent: WO 97/25275 (1997-08-01), None
patent: WO 98/01533 (1998-01-01), None
patent: WO 99/13108 (1999-03-01), None
patent: WO 99/42838 (1999-08-01), None
patent: WO 99/64519 (1999-12-01), None
patent: WO 00/56925 (2000-09-01), None
patent: WO 00/66607 (2000-11-01), None
International Search Report dated May 3, 2001 for International Application No. PCT/US00/29724 entitled, “Tag library compounds, compositions, kits and methods of use.”.
Fitch et al., “Improved Methods for Encoding and Decoding Dialkylamine-Encoded Combinatorial Libraries”, J. Comb. Chem. 1999, 1, 188-194.
Ni et al., “Versatile Approach to Encoding Eombinatorial Organic Synthesis Using Chemically Robust Secondary Amine Tags”, J. Med. Chem. 1996, 39, 1601-1608.
Giese, “Electrophoric Release Tags: Ultrasensitive Molecular Labels Providing Multiplicity”, Trends in Analytical Chemistry, vol. 2, No. 7, 1983, 166-168.
Adam, W. and Liu, J. -C., “Photooxygenation (Singlet Oxygen) of Tetrathioethylenes”J. Am. Chem. 94:1206-1209 (1972).
Adam, W., et al., “Photooxygenation of Vinyl Sulfides: Substituent Effects on the [2+2] Cycloaddition versus Schenck Ene Reaction Modes”Tetrahedron Letters 36(43):7853-7854 (1995).
Ando, W., et al., “Singlet Oxygen Reaction-II alkylthiosubstituted ethylene”Tetrahedron Letters 29:1507-1513 (1973).
Ando, W., et al., “Singlet Oxygen Reaction. III. ‘Solvent and Temperature Effects’ on the Photosensitized Oxygenation of Vinyl Sulfides and Vinyl Ethers”J. Am. Chem. Soc. 96:6766-6768 (1974).
Ando, W., et al., “Singlet Oxygen Reaction. IV. Photooxygenation of Enamies Involving a Two-Step Cleavage of a 1,2-Dioxetane Intermediate”J. Am. Chem. Soc. 97:5028-5029 (1975).
Ando, W., et al., “Singlet Oxygen Reaction V. Ring Size Effects on the Decomposition of Sulfur Substituted 1,2-Dioxetane”Tetrahedron Letters 47:4127-4130 (1975).
Brenner, S. and Lerner, R.A., “Encoded combinatorial chemistry”Proc. Natl. Acad. Sci. USA 89:5381-5383 (1992).
Hacia, J.G., et al., “Detection of heterozygous mutations in BRCA1 using high density ologonucleotide arrays and two-colour fluorescence analysis”Nature Genetics. 14:441-447 (1996).
Haff, L.A. and Smirnov, I.P., “Multiplex genotyping of PCR products with MassTag-labeled primers”Nucleic Acids Res. 25:(18):3749-3750 (1997).
Lee, L.G., et al., “Allelic discrimination by nick-translation PCR with fluorogenic probes”Nucleic Acid Research 21(16):3761-3766 (1993).
Marino, M.A., et al., “Characterization of mitochondrial DNA using low-stringency single specific primer amplification analyzed by laser induced fluorescence-capillary electrophoresis”Electrophoresis 17:1499-1504 (1996).
Matthews, J.A. and Kricka, L.J., “Analytical Strategies for the Use of DNA Probes”Anal. Biochem. 169:1-25 (1988).
Pastinen, T., et al., “Multiplex, fluorescent, solid-phase minisequencing for efficient screening of DNA sequence variation”Clinical Chemistry 42(9):1391-1397 (1996).
Ross, P.L., et al., Discrimination of Single-Nucleotide Polymorphisms in Human DNA Using Peptide Nucleic Acid Probes.
Detected by Maldi-Tof Mass SpectrometryAnal. Chem. 6969:4197-4202 (1997).
Still, W.C., “Discovery of Sequence-Selective Peptide Binding by Synthetic Receptors Using Encoded Combinatorial Libraries”Accounts of Chem. Res. 29:155-163 (1996).
Ullman, E.F., et al., “Luminescent oxygen channeling immunoassay: Measurement of particle binding kinetics by chemiluminescence”Proc. Natl. Acad. Sci., 91:5426-5430 (1994).
Wang, D.G., et al., “Large-Scale Identification, Mapping, and Genotyping of Single-Nucleotide Polymorphisms in the Human Genome”Science 280(5366):1077-1082 (1997).
Wasserman, H.H. and Terao, S., “Enamine-singlet oxygen reactions. α-diketones from intermediate amino dioxetanes”Tetrahedron Letters 21:1735-1738 (1975).
Wetmur, J.G., “DNA Probes: Applications of the Principles of Nucleic Acid Hybridization”Critical Rev. in Biochem. and Molecular Biol. 26(3/4):227-259 (1991).
White, T.J., “The future of PCR technology: diversification of technologies and applications”Trends in Biotechnology 14:478-483 (1996).
Woolley, A. T., et al., “Functional Integration of PCR Amplification and Capillary Electrophoresis in a Microfabricated DNA Analysis Device”Anal. Chem. 68:4081-4086 (1996).
Zalika, K.A., et al., “Mechanisms of 1,2-dioxetane decomposition: the role of electron transfer”Photochem. Photobiol. 30:35-44 (1979).

Affiliated with

Also associated with

Rating

Kits employing generalized target-binding e-tag probes does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Kits employing generalized target-binding e-tag probes, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Kits employing generalized target-binding e-tag probes will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-3649025