Kit for vitrifying specimens

Chemistry: molecular biology and microbiology – Differentiated tissue or organ other than blood – per se – or... – Including freezing; composition therefor

Reexamination Certificate

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C435S260000, C435S307100, C435S975000

Reexamination Certificate

active

07087370

ABSTRACT:
The present invention relates to systems for the vitrification of a biological specimen. The systems employ a transfer instrument such as a loop, containing a base medium that includes a cryoprotectant. The biological specimen which has undergone vitrification may be stored for a period of time, and then thawed at a later date. The thawed biological specimen remains viable. Preferred biological specimens according to the present invention are developmental cells.

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Zhu, S. E. et al., “Cryopreservation of expanded mouse blastocysts by vitrification in ethylene glycol-based solutions,”Journal of Reproduction and Fertility, vol. 98, 1993, pp. 139-145; published by Journals of Reproduction and Fertility.
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