Kit for preparing sample for use in endotoxin test

Details Kit for preparing sample for use in endotoxin test Kit for preparing sample for use in endotoxin test

1983-04-28

1984-10-09

Turk, Arnold

Chemical apparatus and process disinfecting, deodorizing, preser

Analyzer, structured indicator, or manipulative laboratory...

Sample mechanical transport means in or for automated...

210635, 210927, 436502, 436808, 436825, G01N 3348, G01N 3354

Patent

active

044760930

ABSTRACT:
A method for preparing a sample for use in endotoxin test which comprises subjecting a sample of plasma or blood preparation to a contact with water-insoluble anti human .alpha..sub.2 -macroglobulin antibody and to a gel filtration by means of a gel of allyl dextran crosslinked with N,N'-methylenebis acrylamide or a gel of polyvinyl alcohol having many hydrophilic hydroxyl groups, and recovering the maximum molecular weight fraction.
A kit for preparing a sample for use in the endotoxin test consisting of a set of a sample-developing column prepared by packing a water-insoluble anti-human .alpha..sub.2 -macroglobulin antibody and a gel of allyl dextran crosslinked with N,N'-methylenebis acrylamide or a gel of polyvinyl alcohol having many hydrophilic hydroxyl groups, both equilibrated with a buffer solution of pH 7.0-7.4, into a column and tightly sealing the column, an aqueous solution of a high molecular weight colored substance to be added to the sample and a buffer solution for use in developing the sample.

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patent: 4138474 (1979-02-01), Updike
patent: 4273557 (1981-06-01), Juranas
patent: 4276050 (1981-06-01), Firca et al.
patent: 4279774 (1981-07-01), Lindsay et al.
patent: 4350760 (1982-09-01), Nicolas et al.
DuBose et al., Chemical Abstracts, vol. 92, 1980, No. 92:176996t.
McEntire, Chemical Abstracts, vol. 90, 1979, No. 90:70360z.
Gray, Analytical Chemistry, 52, 9R-15R, 1980.
Schenkein et al., "The Journal of Clinical Investigation", vol. 50, 1971, pp. 1864-1868.

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