Chemistry: analytical and immunological testing – Nitrogen containing – Urea or blood urea nitrogen
Patent
1997-09-12
1999-03-30
Alexander, Lyle A.
Chemistry: analytical and immunological testing
Nitrogen containing
Urea or blood urea nitrogen
435 12, 436106, 436120, 436171, 422 61, 422 8205, G01N 3362
Patent
active
058888284
ABSTRACT:
A method of measuring urea nitrogen on the basis of the ultraviolet absorption of urease-GLDH by a reaction rate method, which method not only permits accurate measurement of a sample containing a high concentration of urea nitrogen but also permits accurate measurement of a sample containing polyols such as mannitol without suffering the influence of the polyols, the method comprising hydrolyzing urea in a sample with urease, reacting glutamate dehydrogenase (GLDH) with ammonia formed by the hydrolysis, in the presence of .alpha.-ketoglutaric acid (.alpha.-KG) and reduced-type nicotineamide adenine dinucleotide (NADH) or reduced-type nicotineamide adenine dinucleotide phosphate (NADPH), and measuring the reduced-type nicotineamide adenine dinucleotide (NADH) or reduced-type nicotineamide adenine dinucleotide phosphate (NADPH) for a decrease rate to measure urea nitrogen derived from the urea, and characterized in that a sulfhydryl compound is co-present with the urease.
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English Translation of Japanese Patent JP 59-151900.
English Translation of Japanese Patent JP 02255099.
English Translation of Japanese Patent JP 03065160.
Katayama Katsuhiro
Kojima Ryo
Tanaka Hirotoshi
Alexander Lyle A.
Carrillo S.
Nitto Boseki Co. Ltd.
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