Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Reexamination Certificate
2010-10-06
2011-11-22
Gabel, Gail R (Department: 1641)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
C435S007210, C435S287200, C435S334000, C435S355000, C435S973000, C436S521000, C436S522000, C436S010000, C436S017000, C436S063000, C436S094000, C436S172000, C436S175000, C436S811000, C422S073000, C422S082080, C422S430000, C424S009200
Reexamination Certificate
active
08062860
ABSTRACT:
The present invention relates a method for the enumeration of in vivo gene mutation. The method utilizes differential staining of GPI-anchor deficient erythrocyte populations to distinguish between wild-type and pig-a gene mutants. Quantitative analyses can be conducted on erythrocytes and/or reticulocytes, and is based upon fluorescent emission and light scatter following exposure to an excitatory light source. Counting of mutant erythrocytes or reticulocytes relative to the number of total erythrocytes or reticulocytes can be used to assess the DNA-damaging potential of an exogenous chemical agent, the DNA-damaging potential of an exogenous physical agent, the effects of an exogenous agent which can modify endogenously-induced DNA damage, and the effects of an exogenous agent which can modify exogenously-induced DNA damage. Kits for practicing the invention are also disclosed.
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Gabel Gail R
LeClairRyan, a Professional Corporation
Litron Laboratories, Ltd.
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