Kit for luciferase assay

Details Kit for luciferase assay Kit for luciferase assay

1995-06-07

1997-06-24

Tsang, Cecilia

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving luciferase

435 4, 435184, 435810, 435968, 435975, 436164, 436166, 436172, 436808, C12Q 166, G01N 3353

Patent

active

056416415

ABSTRACT:
Methods and compositions are provided for improved kinetics of light production from luciferase activity in beetle luciferase-luciferin reactions. Thus, the invention provides methods, compositions and test kits for assaying samples for the presence of a beetle luciferase or, using a beetle luciferase-luciferin reaction, the presence of ATP. The invention also provides the complex of the coenzyme, Coenzyme A, a beetle luciferase and oxyluciferin in its exited state in the luciferase-luciferin reaction and luciferyl-CoA, the thioester of Coenzyme A and luciferin (D-(-)-2-(6'-hydroxy -2'-benzothiazolyl)-.DELTA..sup.2 -thiazoline-4-carboxylic acid).

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