Chemistry: electrical and wave energy – Processes and products – Electrostatic field or electrical discharge
Patent
1988-05-02
1990-08-14
Niebling, John F.
Chemistry: electrical and wave energy
Processes and products
Electrostatic field or electrical discharge
2041801, 204299R, G01N 2728, G01N 2726, B01D 5702
Patent
active
049484800
ABSTRACT:
A polyacrylamide type gel medium for electrophoresis is conveniently and safely prepared prior to use from a kit which comprises (1) a water-soluble copolymer of an acrylamide monomer and at least one comonomer copolymerizable therewith, which comonomer contains functional groups that permit crosslinking of said copolymer by a covalent but non-free radical initiated mechanism; and (2) a selected crosslinking agent in a package that separates it from the copolymer. Optionally, additional materials that may be usefully incorporated in the gel may be included in the kit, for example, a selected electrophoresis buffer and a complexing agent, such as SDS. The kit is used to prepare the gel by forming a solution of the copolymer in deionized water at the desired concentration of copolymer, adding a sufficient amount of any optional ingredients to provide the desired concentrations thereof, adding such concentration of the crosslinking agent as will provide a gelation time of between about 5 minutes and about 15 hours, and pouring the so prepared reaction mixture into a preselected mold or casting device or onto a support so as to form the resulting gel into the desired shape and size.
REFERENCES:
patent: 4215195 (1980-07-01), Ponticello et al.
patent: 4346231 (1982-08-01), Ponticello et al.
patent: 4582868 (1986-04-01), Ogawa et al.
patent: 4704198 (1987-11-01), Ebersole et al.
U. K. Laemmli, "Cleavage of Structural Proteins During the Assembly of the Head of Bacteriophage T4," Nature, 1970, vol. 227, pp. 680-685.
Christy, Jr. Kenneth G.
Osterhoudt Hans W.
Ponticello Ignazio S.
Deaton Betty J.
Eastman Kodak Company
Niebling John F.
Starsiak Jr. John S.
LandOfFree
Kit for electrophoresis gel medium does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Kit for electrophoresis gel medium, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Kit for electrophoresis gel medium will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-460734