Kit for electrophoresis gel medium

Chemistry: electrical and wave energy – Processes and products – Electrostatic field or electrical discharge

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

2041801, 204299R, G01N 2728, G01N 2726, B01D 5702

Patent

active

049484800

ABSTRACT:
A polyacrylamide type gel medium for electrophoresis is conveniently and safely prepared prior to use from a kit which comprises (1) a water-soluble copolymer of an acrylamide monomer and at least one comonomer copolymerizable therewith, which comonomer contains functional groups that permit crosslinking of said copolymer by a covalent but non-free radical initiated mechanism; and (2) a selected crosslinking agent in a package that separates it from the copolymer. Optionally, additional materials that may be usefully incorporated in the gel may be included in the kit, for example, a selected electrophoresis buffer and a complexing agent, such as SDS. The kit is used to prepare the gel by forming a solution of the copolymer in deionized water at the desired concentration of copolymer, adding a sufficient amount of any optional ingredients to provide the desired concentrations thereof, adding such concentration of the crosslinking agent as will provide a gelation time of between about 5 minutes and about 15 hours, and pouring the so prepared reaction mixture into a preselected mold or casting device or onto a support so as to form the resulting gel into the desired shape and size.

REFERENCES:
patent: 4215195 (1980-07-01), Ponticello et al.
patent: 4346231 (1982-08-01), Ponticello et al.
patent: 4582868 (1986-04-01), Ogawa et al.
patent: 4704198 (1987-11-01), Ebersole et al.
U. K. Laemmli, "Cleavage of Structural Proteins During the Assembly of the Head of Bacteriophage T4," Nature, 1970, vol. 227, pp. 680-685.

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Kit for electrophoresis gel medium does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Kit for electrophoresis gel medium, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Kit for electrophoresis gel medium will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-460734

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.