Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving luciferase
Patent
1997-03-17
1998-09-22
Naff, David M.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving luciferase
210295, 210615, 435 29, 435 39, 435243, 435291, 435297, 435300, 435301, 435975, C02F 300, C12M 134, C12N 100, G01N 3353
Patent
active
058112510
ABSTRACT:
A rapid, convenient, and highly sensitive method and kit for counting the number of living microorganisms are disclosed. A test solution is filtrated through a membrane filter element having a plurarity of hydrophilic sections substantially perfectly separated from each other by hydrophobic partitions. A microparticulate spray is then applied to said filter in order to add extracting and luminescence-inducing reagents in such an amount that allows the filter membrane within each section to become wet. Thereafter the sample obtained is processed by applying a highly sensitive bioluminescence image analyzing system thereto. The employment of a spray method for applying the extracting and luminescence-inducing reagents allows to substantially perfectly entrap the reagents and extracted substances within each of the hydrophilic sections, thus enabling, when combined with the use of a bioluminescence image analyzing system, to rapidly count the number without any or substantial requirement of cultivation.
REFERENCES:
patent: 3616253 (1971-10-01), D'Eustachio et al.
patent: 3929583 (1975-12-01), Sharpe et al.
patent: 4587213 (1986-05-01), Malecki
patent: 4618533 (1986-10-01), Steuck
patent: 4908236 (1990-03-01), Pitt et al.
patent: 5366867 (1994-11-01), Kawakami et al.
patent: 5627042 (1997-05-01), Hirose et al.
Tsai, T.S., et al., "Rapid Separation and Quantitation of Mixed Microorganisms by Filtration and Bioluminescence (42388)," Proceedings of the Society for Experimental Biology and Medicine, 183:74-80 May 1986.
Rodrigues, U.M. and Kroll, R.G., "The direct epifluorescent filter technique (DEFT): increased selectivity, sensitivity and rapidity," Journal of Applied Bacteriology, 59:493-500 Jul. 1985.
Hirose, deceased Atsumi
Kataoka Takuji
Seto Susumu
Wang David
Naff David M.
Nihon Millipore Kabushiki Kaisha
Ware Deborah K.
LandOfFree
Kit for counting the number of living microorganisms does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Kit for counting the number of living microorganisms, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Kit for counting the number of living microorganisms will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1621026