Kit for converting a standard microscope into a single aperture

Details Kit for converting a standard microscope into a single aperture Kit for converting a standard microscope into a single aperture

1990-11-30

1993-08-10

Arnold, Bruce Y.

Optical: systems and elements

Compound lens system

Microscope

359227, G02B 2100, G02B 2602

Patent

active

052354571

ABSTRACT:
A kit to convert a standard epi-illumination microscope into a single aperture confocal scanning epi-illumination microscope comprises an assembly including, in one embodiment, a pair of intermediate lenses to create a second field plane conjugate to the specimen plane in both the incident and reflected light paths, with a single aperture positioned at this second conjugate field plane and controllably scanned through the plane to create the incident light beam as well as to mask the returning light before viewing. In a second embodiment, only a single lens is included in the assembly and the objective lens may be positioned at its correct tube length, or a non-standard objective lens may be used to prevent undesired degradation of the image. This assembly conveniently mounts to the nosepiece of the standard microscope in place of the objective lens, and the objective lens mounts to the assembly.

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Amos et al, "Use of confocal imaging in the study of biological structures", Applied Optics, vol. 26, No. 16, Aug. 15, 1987, pp. 3239-3243.
Xiao et al, "Real-time confocal scanning optical microscope", Appl. Phys. Lett. vol. 53, No. 8, Aug. 22, 1988.

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