Kit for conducting an assay to detect a substance using enzymati

Details Kit for conducting an assay to detect a substance using enzymati Kit for conducting an assay to detect a substance using enzymati

1998-10-30

2000-09-05

Schwartzman, Robert A.

Compositions

Chemiluminescent

435183, 549510, C09K 300

Patent

active

061138169

ABSTRACT:
A kit for detecting a first substance in a sample comprising a dioxetane having the formula: ##STR1## wherein T is a cycloalkyl or polycycloalkyl group bonded to the 4-membered ring portion of the dioxetane by a spiro linkage; Y is a fluorescent chromophore; X is H, alkyl, aryl, aralkyl, alkaryl, heteroalkyl, heteroaryl, cycloalkyl, cycloheteroalkyl, or an enzyme-cleavable group; and Z is H or an enzyme-cleavable group, provided that at least one of X or Z must be an enzyme-cleavable group; and an enzyme which cleaves the enzyme-cleavable group of the dioxetane creating an electron-rich moiety which destabilizes the dioxetane, causing it to decompose into two ketones, one ketone comprising the moiety T, and the other ketone comprising moieties X, Y and a portion of Z. The energy released by decomposition causes the moiety Y to luminesce.

REFERENCES:
patent: 4857652 (1989-08-01), Schaap
patent: 4956477 (1990-09-01), Bronstein et al.
patent: 4962192 (1990-10-01), Schaap
patent: 4978614 (1990-12-01), Bronstein
patent: 5177241 (1993-01-01), Bronstein et al.
patent: 5225584 (1993-07-01), Brooks et al.
patent: 5330900 (1994-07-01), Bronstein et al.
patent: 5362916 (1994-11-01), Edwards et al.
patent: 5538847 (1996-07-01), Bronstein et al.
patent: 5582980 (1996-12-01), Bronstein et al.
patent: 5605795 (1997-02-01), Bronstein et al.
patent: 5625077 (1997-04-01), Bronstein
patent: 5637747 (1997-06-01), Bronstein et al.
patent: 5639907 (1997-06-01), Bronstein et al.
patent: 5648555 (1997-07-01), Bronstein et al.
patent: 5679802 (1997-10-01), Bronstein et al.
patent: 5756770 (1998-05-01), Bronstein et al.
patent: 5763681 (1998-06-01), Edwards et al.
patent: 5777133 (1998-07-01), Bronstein et al.
patent: 5856522 (1999-01-01), Bronstein et al.
patent: 5866389 (1999-02-01), Bronstein

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