Kit for conducting an assay to detect a substance using...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

Reexamination Certificate

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

C549S510000

Reexamination Certificate

active

06913897

ABSTRACT:
In an assay method in which a member of a specific binding pair is detected by means of an optically detectable reaction, the improvement wherein the optically detectable reaction includes the reaction, with an enzyme, of a dioxetane having the formulawhere T is a cycloalkyl or polycycloalkyl group bonded to the 4-membered ring portion of the dioxetane by a spiro linkage; Y is a fluorescent chromophore; X is H, alkyl, aryl, aralkyl, alkaryl, heteroalkyl, heteroaryl, cycloalkyl, cycloheteroalkyl, or enzyme-cleavable group; and Z is H or an enzyme-cleavable group, provided that at least one of X or Z must be an enzyme-cleavable group, so that the enzyme cleaves the enzyme-cleavable group from the dioxetane to form a negatively charged substituent bonded to the dioxetane, the negatively charged substituent causing the dioxetane to decompose to form a luminescent substance that includes group Y of said dioxetane.

REFERENCES:
patent: 2478377 (1949-08-01), Dickey
patent: 3009962 (1961-11-01), Milas
patent: 3442813 (1969-05-01), Bollyky
patent: 3442814 (1969-05-01), Sheehan
patent: 3442815 (1969-05-01), Rauhut
patent: 3470103 (1969-09-01), Sheehan
patent: 3597362 (1971-08-01), Bollyky
patent: 3677957 (1972-07-01), Moulding
patent: 3720622 (1973-03-01), Bollyky
patent: 3763188 (1973-10-01), Krespan
patent: 3862142 (1975-01-01), Story
patent: 4302534 (1981-11-01), Halmann
patent: 4315998 (1982-02-01), Neckers et al.
patent: 4372745 (1983-02-01), Mandle
patent: 4446233 (1984-05-01), Auditore-Hargreave
patent: 4604364 (1986-08-01), Kosak
patent: 4663278 (1987-05-01), DiNello
patent: 4665018 (1987-05-01), Vold
patent: 4857652 (1989-08-01), Schaap
patent: 4956477 (1990-09-01), Bronstein et al.
patent: 4962192 (1990-10-01), Schaap
patent: 4978614 (1990-12-01), Bronstein et al.
patent: 5177241 (1993-01-01), Bronstein et al.
patent: 5225584 (1993-07-01), Brooks et al.
patent: 5330900 (1994-07-01), Bronstein et al.
patent: 5336596 (1994-08-01), Bronstein et al.
patent: 5362916 (1994-11-01), Edwards et al.
patent: 5538847 (1996-07-01), Bronstein et al.
patent: 5547836 (1996-08-01), Bronstein et al.
patent: 5582980 (1996-12-01), Bronstein et al.
patent: 5605795 (1997-02-01), Bronstein et al.
patent: 5625077 (1997-04-01), Bronstein
patent: 5637747 (1997-06-01), Bronstein et al.
patent: 5639907 (1997-06-01), Bronstein et al.
patent: 5648555 (1997-07-01), Bronstein et al.
patent: 5654154 (1997-08-01), Bronstein et al.
patent: 5679802 (1997-10-01), Bronstein et al.
patent: 5753436 (1998-05-01), Bronstein et al.
patent: 5756770 (1998-05-01), Bronstein et al.
patent: 5763681 (1998-06-01), Edwards et al.
patent: 5777133 (1998-07-01), Bronstein et al.
patent: 5847161 (1998-12-01), Bronstein et al.
patent: 5849495 (1998-12-01), Bronstein et al.
patent: 5856522 (1999-01-01), Bronstein et al.
patent: 5866389 (1999-02-01), Bronstein et al.
patent: 5869699 (1999-02-01), Bronstein et al.
patent: 5869705 (1999-02-01), Bronstein
patent: RE36536 (2000-01-01), Bronstein et al.
patent: 6113816 (2000-09-01), Bronstein et al.
patent: 6124478 (2000-09-01), Bronstein et al.
patent: 6287767 (2001-09-01), Bronstein et al.
patent: 6322727 (2001-11-01), Bronstein et al.
patent: 6514717 (2003-02-01), Bronstein et al.
patent: 1044639 (1979-12-01), None
patent: 2383404 (1978-10-01), None
patent: 57/042686 (1982-03-01), None
Geller, et al., Tetrahedron Letters, 673 (1983).
Adam, et al., Chem. Ber., 116, 839 (1983).
Patolia and Trivedi, Indian J. Chem., 22B 444 (1983).
McMurry et al., J. Am. Chem. Soc., 105 pp. 1660-1661 (1983).
Schaap et al., Tetrahedron Letters, 2943 (1982).
Schaap et al., J. Amer. Chem. Soc., 104, 3504 (1982).
Wynberg et al., In Bioluminescence & Chemiluminescence, DeLuca and McElroy (eds.) Academic Press, New York, p. 687 (1981).
Shimomura, et al., Photocham. Photobiol., 30, 89 (1979).
Schaap et al., J. Amer. Chem. Soc., 101, 4016 (1979).
Zaklika et al., Photochem. Photobiol., 30, 35 (1979).
McMurry et al., J. Org. Chem., vol. 43, No. 17, 3255-3266 (1978).
Zaklika et al., J. Amer. Chem. Soc., 100, 318 (1978).
Zaklika et al., J. Amer. Chem. Soc., 100, 4916 (1978).
McMurry et al., J. Org. Chem., vol. 42, No. 15, 2655-2656 (1977).
McCapra et al., J. Chem. Soc. Chem. Commun., 944 (1977).
Schaap et al., J. Amer. Chem. Soc., 99, 12790 (1977).
Corey et al., J. Amer. Chem. Soc., 94, 6190 (1972).
Wilson, et al., J. Amer. Chem. Soc., 93, 4126 (1971).
Bartlett et al., J. Amer. Chem. Soc., 92, 3223 (1970).
Mazur et al., J. Amer. Chem. Soc., 92, 3225 (1970).
Schaap et al., Organic Photochemical Synthesis, II, 49 (1976).
Schaap et al., J. Amer. Chem. Soc., 97, 7110 (1975).
Turro et al., J. Amer. Chem. Soc., 97, 7110 (1975).
Wieringa et al., Tetrahedron Letters, 169 (1972).
Kopecky et al., Can. J. Chem., 47, 709 (1969).
McCapra, F., Chem. Commun., 155 (1968).
Davies et al., J. Org. Chem., 23, 307 (1958).
Sunthankar et al., J. Org. Chem., 16, 8 (1951).
Handwritten notes from Dr. Charles Zepp, prepared sometime in Apr. 1984.
Handwritten notes from S. Stroud to C. Zepp (Polaroid Corp.), dated Jul. 2, 1984.
Handwritten notes of Dr. Charles Zepp (Polaroid dioxetane file), 1984.
Handwritten notes of Dr. Charles Zepp, 1984.
Presentation sheet, Dr. Charles Zepp (Polaroid Corp.).
Handwritten notes and data plot from Dr. A. Paul Schaap to Dr. I.Y. Bronstein, dated Nov. 13, 1985.
Disclosure and cover letter from Dr. A.P. Schaap to Donald H. Siegal, Esq., Jun. 1986.
Materials from Dr. A. Paul Schaap, Dec. 2, 1985.
Complaint:Quest systems, Inc.v.C. Zeppin Massachusetts State Court.
C. Zepp's Answer to Quest's Complaint.
Complaint:Wayne State Universityv.Quest Systemsin Michigan State Court.
Complaint:Quest Systemsv.Wayne State Universityin Massachusetts State Court.
Complaint:Wayne State Universityv.Quest Systemsin U.S. District Court.
Nicolas, et al., Ann. Biol. Clin. (Paris) 43:201-5 (1985).
Handwritten notes of Paul Schaap (Jul. 20, 1983).
Hummelen et al., CA 100: 209242h (1984).
Adam et al., Tet. Lett. 23: 2835-36 (1982).
Meijer et al., Tet. Lett. 41: 3997-4000 (1979).
Wynberg et al., “1,2-Dioxetanes as Chemiluminescent Probes and Labels,” in bioluminescence and chemiluminescence. Basic chemistry and Analytical applications, ed. By M.A. DeLuca et al. (1981).
Affidavit of Charles M. Zepp, submitted as attachment to paper 13 in great grandparent U.S. Appl. No. 06/889,823.
Zeitschrift fur Naturforschung 39b (5), May 1984, p 679-682 by Waldenmar Adam. Tubingen FRG Q 324.
Handley et al., Tetrahedron Lett., 3183 (1985).
Kricka, L.J., Ligand-binder Assays, Marcel Bekker, Inc., New York, p. 170 (1985).
Kricka, L.J., Ligand-Binder Assays, Marcel Dekker, Inc., New York, p. 199 (1985).

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Kit for conducting an assay to detect a substance using... does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Kit for conducting an assay to detect a substance using..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Kit for conducting an assay to detect a substance using... will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-3378575

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.