Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving transferase
Patent
1989-12-27
1993-06-01
Naff, David M.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving transferase
435975, 435 13, 435810, 435 4, C12Q 100, C12Q 156, C12Q 148
Patent
active
052158888
ABSTRACT:
There is provided a kit or reagent for assaying the cellular integrity (intactness) of blood platelets comprising means for determining the quantity of PKA released by a number of platelets into a certain volume of plasma. The assay is based on determining cAMP-dependent phosphorylation of vitronectin (protein S) in plasma using radioactively labelled [.gamma..sup.32 P]ATP as phosphate donor. The assay can be based on the specific inhibition of the phosphorylation in blood fluid by protein kinase inhibitors. The invention further relates to pharmaceutical compositions for the treatment of pathological conditions associated with impaired platelet function in humans, which comprises administering to a person in need thereof a physiologically active quantity of a PKA inhibitor adapted to decrease or prevent vitronectin phosphorylation. The inhibitor can be synthetic or genetically engineered peptide.
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Korc-Grodzicki, B. et al (1988), "Platelet stimulation releses a cAMP-dependent protein kinase that specifically phosphorylates a plasma protein", Proc. Natl. Acad. Sci. U.S.A., vol. 85, pp. 7541-7545.
Suzuki, S. et al (1984), "Domain structure of vitronectin", The Journal of Biological Chemistry, vol. 259, pp. 15307-15314.
Korc-Grodzicki, B. et al (1988), "Vitronectin is phosphorylated by a cAMP-dependent protein kinase released by activation of human platelets with thrombin", Biochemical and Biophysical Research Communications, vol. 157, No. 3, pp. 1131-1138.
Chain Daniel
Grodzicki Beatriz
Shaltiel Shmuel
Meller Mike
Naff David M.
Yeda Research and Development Co. Ltd.
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