Kit for assaying acetyl transferase or deacetylase activity

Details Kit for assaying acetyl transferase or deacetylase activity Kit for assaying acetyl transferase or deacetylase activity

2009-10-13

2010-12-14

Gitomer, Ralph (Department: 1657)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving transferase

C435S024000, C435S975000, C530S345000

Reexamination Certificate

active

07851175

ABSTRACT:
The invention provides methods and kits for characterizing the activity of an acetyl transferase or deacetylase. The method involves enzymatically acetylating or deacetylating in vitro a substrate that is a peptide fragment of a full-length polypeptide, and then non-enzymatically acylating the peptide substrate with acyl groups that differ in molecular weight from the enzymatically added or removed acetyl groups. Typically, deuterated acetic anhydride is used to non-enzymatically acylate the substrate. The fully acylated substrate is then characterized by mass spectrometry to determine the amino acid positions of the substrate that are enzymatically acetylated or deacetylated.

REFERENCES:
patent: 7670795 (2010-03-01), Tackett et al.
patent: 2003/0224473 (2003-12-01), McCafferty
patent: 2004/0091951 (2004-05-01), Schultz
patent: 2006/0111287 (2006-05-01), Bianchi

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