Kinetic method for directly determining total bilirubin

Details Kinetic method for directly determining total bilirubin Kinetic method for directly determining total bilirubin

1980-04-21

1982-01-19

Marcus, Michael S.

Chemistry: physical processes

Physical processes

Crystallization

23905, G01N 3350, G01N 3372, G01N 3352

Patent

active

043114830

ABSTRACT:
A kinetic method for directly assaying total bilirubin in a sample. The kinetic assay entails mixing the sample to be assayed with an azo reagent and measuring the rate of formation of azobilirubin, wherein the rate of azobilirubin formation is directly proportional to the concentration of bilirubin in the sample being assayed. The azo reagent comprises a diazotized aromatic amine and accelerator and the composition and pH thereof is selected such that the rate constants for the conjugated and unconjugated reactions are approximately equal and such that the half-lives of both reactions are greater than 1 second and less tha 5 minutes.

REFERENCES:
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patent: 3923459 (1975-12-01), Ertingshausen et al.
patent: 4038031 (1977-07-01), Lam
patent: 4119401 (1978-10-01), Sansur et al.
Landis et al., Clin. Chem. 24 (10) pp. 1700-1707, and 1690-1699 (1978).
Groso et al., Clin. Chem. 22 (4) 429-433 (1976).

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