Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving transferase
Patent
1977-12-12
1979-07-03
Wiseman, Thomas G.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving transferase
G01N 3114, G01N 3316
Patent
active
041599233
ABSTRACT:
A kinetic assay is provided for determining the inorganic phosphate content in an aqueous solution by measuring the amount of reduced coenzyme produced. The assay is based on the following coupled reactions:
I. maltose+PO.sub.4.sup.-3 maltose phosphorylase glucose+.beta.-D-glucose-1-phosphate;
Ii. .beta.-d-glucose-1-phosphate .beta.-D-phosphoglucomutase glucose-6-phosphate;
Iii. glucose-6-phosphate+NAD glucose-6-phosphate dehydrogenase NADH+6-phosphogluconate.
REFERENCES:
patent: 3977944 (1976-08-01), Muller-Matthesras et al.
patent: 4000042 (1976-12-01), Adams
Kamogawa, et al., "An Enzymatic Method for the Determination of Maltose in the Presence of Other Oligosaccharrides", Anal. Biochem., vol. 57, pp. 303-305 (1974).
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Bergmeyer, Methods of Enzymatic Analysis, vol. 1, Academic Press, New York, 1974, pp. 109-110 and 123-127.
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Morgan, et al., "Substrate Specificity of Maltose Phosphorylase", Nature, vol. 196, pp. 168-169 (1962).
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Kamogawa, et al., "Purification and Properties of Maltose Phosphorylase from Lactobacillus brevis", Arg. Biol. Chem., vol. 37, pp. 2813-2819 (1973).
Fitting et al., "Phosphorolysis of Maltose by Enzyme Preparations from Neiserra Meningitidis", J. Biol. Chem., vol. 199, pp. 153-163 (1952).
Ben-Zui et al., "A Phosphoglucomutase for Beta-glucose-1-phosphate", Biochem. Biophys. Res. Com. vol. 2, NO. 1, pp. 8-11 (1960).
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Ben-Zui et al., "A Phosphoglucomutase Specific for Beta-glucose-1-phosphate", J. Bio. Chem., vol. 236, pp. 2186-2189 (1961).
Nadj Henriette
Pierre Kenneth J.
Tung Ker-Kong
Beckman Instruments Inc.
Frieman Robert S.
Steinmeyer Robert J.
Wiseman Thomas G.
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