Kinase assay

Details Kinase assay Kinase assay

1996-08-26

1998-06-02

Loring, Susan A.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435 75, 435 4, 435968, 436517, G01N 33573, G01N 3353, G01N 33543

Patent

active

057597871

ABSTRACT:
The invention provides novel methods and compositions for detecting kinase activity in solution, without the use of radioactivity. The methods marry the kinetic advantages of solution-based reaction with the efficiency and high-throughput adaptability of solid-phase wash and detection steps, yet is conveniently practiced in a single tube. The methods may be used to assay for kinase activity per se or, by controlling for the kinase activity, for modulators of kinase activity. The method is exemplified with a preferred chemiluminescent protein kinase assay using biotinylated substrate peptides captured on a streptavidin coated microtiter plate and monoclonal antibodies to detect their phosphorylation.

REFERENCES:
patent: 5223402 (1993-06-01), Abbas
patent: 5441894 (1995-08-01), Colemena et al.
patent: 5610016 (1997-03-01), Sato et al.
patent: 5641635 (1997-06-01), Emmons et al.
patent: 5657118 (1997-08-01), Lee
Yano, T. et al. A Monoclonal Antibody to the Phosphorylated Form of Glial Fibrillary Acidic Protein: Application to a Non-Radioactive Method for Measuring Protein Kinase Activities. Biochem. Res. Com. 29 Mar. 1991, vol. 175, No. 3, p. 1144-1151.

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