Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for...
Patent
1993-05-07
1995-01-03
Knode, Marian
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
435171, 435 711, 435814, 435911, 435917, 435942, 4352568, 4352541, 530412, 530823, 530824, C12N 900, C12P 2100
Patent
active
053786211
ABSTRACT:
This invention provides a method for killing fungal cells without lysing in fermentation processes in order to prepare the fermentation mixture for processing to recover or extract an extracellularly expressed enzyme from the fermentation mixture. A preferred method of this invention comprises adjusting the pH of the fermentation mixture to less than 2.79 using a mineral acid, then adding sufficient acetic acid to the mixture to affect a substantially complete cell kill in mixture. A salt of the acetic acid can be used. The organic acid or salt can be added, then the pH adjusted to the desired level. Other organic acids can be used, in which case the pH of the mixture is adjusted to the pK.sub.a of the selected organic acid before the organic acid is added to the mixture. The method of this invention is useful for stopping the growth and killing the cells in any micro-organism, culture or fermentation such as those containing yeast, bacteria or fungi and is particularly useful in systems where it is desired to kill the cells without lysing them.
REFERENCES:
patent: 1908225 (1933-05-01), Currie et al.
patent: 3134723 (1964-05-01), Corman
patent: 3345268 (1967-10-01), Corman
patent: 3779868 (1973-12-01), Nikolaev et al.
patent: 3816260 (1974-06-01), Sugiyama
patent: 3890198 (1975-06-01), Kobayashi et al.
patent: 3917510 (1975-11-01), Kitamura et al.
patent: 3961080 (1976-06-01), Sugimoto et al.
patent: 4299858 (1981-11-01), Aubert et al.
patent: 4596778 (1986-06-01), Hitzman
patent: 4601986 (1986-07-01), Wegner et al.
patent: 4647458 (1987-03-01), Ueno et al.
patent: 4654306 (1987-03-01), Entani et al.
patent: 4725544 (1988-02-01), Jan et al.
patent: 4935360 (1990-06-01), Klemps et al.
patent: 5155040 (1992-10-01), Kula et al.
Rusul, G. et al., "Growth and Aflotoxin Production by Aspergillus parasiticis NRRL2999 in The Presence of Acetic or Propionic Acid and at Different Initial pH Values," J. Food Protection, 1987 vol. 50, No. 11, pp. 900 914 919.
Hentges D. J., "Influence of pH on the Inhibitory Activity of Formic and Acetic Acids for Shigella" J. Bacteriology, vol. 93, No. 6, pp. 2029-2030, 1967.
Baliu Enrique F.
Heinsohn Henry G.
Lawlis, Jr. Virgil B.
Genencor, Inc.
Knode Marian
LandOfFree
Killing cells without lysis in a method for enzyme recovery from does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Killing cells without lysis in a method for enzyme recovery from, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Killing cells without lysis in a method for enzyme recovery from will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2211027