KEX2 endoprotease without a C-terminal hydrophobic region

Details KEX2 endoprotease without a C-terminal hydrophobic region KEX2 endoprotease without a C-terminal hydrophobic region

1989-01-31

1992-11-10

Wax, Robert A.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

435219, 4351723, C12N 960, C12N 1500, C12N 950

Patent

active

051622203

ABSTRACT:
A KEX2 endoprotease produced by a recombinant DNA technique, a KEX2 endoprotease shortened at the C-terminal of a native enzyme and still containing a C-terminal hydrophobic region, a soluble KEX2 endoprotease without a C-terminal hydrophobic region; DNA's coding for the above-mentioned enzymes; expression plasmids containing the DNA; hosts transformed with the plasmid; a process for production of the above-mentioned enzymes using the transformed host; and a process for production of biologically active polypeptide using the above-mentioned enzyme.

REFERENCES:
R. S. Fuller et al., Journal of Cellular Biochemistry, Supplement 12B, Jan. 30, 1988, p. 271, Abstract No. X015.
Fuller et al, "The Saccharomyces cerevisiae KEX2 Gene, Required For Processing Prepro-.alpha.-Factor, Encodes a Calcium-Dependent Endopeptidase That Cleaves After Lys-Arg and Arg-Arg Sequences," Microbiology, pp. 273-278, 1986.
Julius et al, "Isolation of the Putative Structural Gene For the Lysine-Argine-Cleaving Endopeptidase Required For Processing of Yeast Prepro-.alpha.-Factor," Cell, 37, pp. 1075-1089, 1984.
Wickner et al, "Two Chromosomal Genes Required For Killing Expression In Killer Strains of Saccharomyces cerevisiae," Genetics, 82, pp. 429-442, 1976.
Mizuno et al, "A Membrane-Bound, Calcium-Dependent Protease In Yeast .alpha.-Cell Cleaving On the Carboxyl Side of Paired Basic Residues," BBRC, 144, pp. 807-814, 1987.
Leibowitz et al, "A Chromosomal Gene Required For Killer Plasmid Expression, Mating, and Spore Maturation In Saccharomyces cerevisiae, " PNAS, 73, pp. 2061-2065, 1976.
Bathurst et al, "Yeast KEX2 Protease Has the Properties of a Human Proalbumin Converting Enzyme," Science, 235, pp. 348-350, 1987.
Fuller et al, "Enzymes Required For Yeast Prohormone Processing," Ann. Rev. Physiol., pp. 345-362, 1988.
Thomas et al, "Yeast KEX2 Endopeptidase Correctly Cleaves a Neuroendocrine Prohormone In Mammalian Cells," Science, 241, pp. 226-230, 1988.
Mizuno et al, "Yeast KEX2 Gene Encodes An Endopeptidase Homologous to Subtilisin-Like Serine Protease," BBRC, 156, pp. 246-254, 1988.

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