Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Oxidoreductase
Reexamination Certificate
2011-07-12
2011-07-12
Raghu, Ganapa Thirama (Department: 1652)
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Oxidoreductase
C435S190000, C435S025000, C435S117000, C435S121000, C435S135000, C536S023200
Reexamination Certificate
active
07977078
ABSTRACT:
The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize chiral compounds.
REFERENCES:
patent: 4443373 (1984-04-01), Girijavallabhan et al.
patent: 4530793 (1985-07-01), Girijavallabhan et al.
patent: 4619924 (1986-10-01), Hamanaka
patent: 4695626 (1987-09-01), Brighty
patent: 4739047 (1988-04-01), Volkmann et al.
patent: 4864046 (1989-09-01), Volkmann
patent: 4874877 (1989-10-01), Urban
patent: 4940823 (1990-07-01), Volkmann
patent: 4954647 (1990-09-01), Urban
patent: 5013729 (1991-05-01), Volkmann
patent: 5191077 (1993-03-01), Volkmann
patent: 5200335 (1993-04-01), Hummel et al.
patent: 5206399 (1993-04-01), Sayo et al.
patent: 5225339 (1993-07-01), Wong et al.
patent: 5319103 (1994-06-01), Volkmann
patent: 5326884 (1994-07-01), Urban
patent: 5342767 (1994-08-01), Wong et al.
patent: 5427933 (1995-06-01), Chen et al.
patent: 5491077 (1996-02-01), Chartrain et al.
patent: 5559030 (1996-09-01), Matsuyama et al.
patent: 5700670 (1997-12-01), Yamagishi et al.
patent: 5891685 (1999-04-01), Yamagishi et al.
patent: 6037158 (2000-03-01), Hummel et al.
patent: 6225099 (2001-05-01), Hummel et al.
patent: 6399339 (2002-06-01), Wolberg et al.
patent: 6413750 (2002-07-01), Hummel et al.
patent: 6645746 (2003-11-01), Kizaki et al.
patent: 6689591 (2004-02-01), Muller et al.
patent: 6800477 (2004-10-01), Patel et al.
patent: 7052885 (2006-05-01), Poechlauer et al.
patent: 7083962 (2006-08-01), Kimoto et al.
patent: 2002/0061564 (2002-05-01), Rozzell
patent: 2003/0054520 (2003-03-01), Bommanus et al.
patent: 2003/0068811 (2003-04-01), Patel et al.
patent: 2004/0265978 (2004-12-01), Gupta et al.
patent: 2006/0195947 (2006-08-01), Davis et al.
patent: 2006/0286646 (2006-12-01), Patel et al.
patent: 2008/0248539 (2008-10-01), Giver et al.
patent: 2008/0318295 (2008-12-01), Ching et al.
patent: 2009/0155863 (2009-06-01), Liang et al.
patent: 2009/0162909 (2009-06-01), Campopiano et al.
patent: 2009/0191605 (2009-07-01), Liang et al.
patent: 2010/0055751 (2010-03-01), Voladri et al.
patent: 2010/0062499 (2010-03-01), Mundorff et al.
patent: 1323830 (2003-07-01), None
patent: 1582518 (2005-10-01), None
patent: 1712633 (2006-10-01), None
patent: WO 01/40450 (2001-06-01), None
patent: WO 02/086126 (2002-10-01), None
patent: WO 2005/017135 (2005-02-01), None
patent: WO 2005/018579 (2005-03-01), None
patent: WO 2005/033094 (2005-04-01), None
patent: WO 2005/054491 (2005-06-01), None
patent: WO 2007/010944 (2007-01-01), None
patent: WO 2007/012428 (2007-02-01), None
patent: WO 2008/042876 (2008-04-01), None
patent: WO 2008/103248 (2008-08-01), None
Broun et al., Catalytic plasticity of fatty acid modification enzymes underlying chemical diversity of plant lipids. Science, 1998, vol. 282: 1315-1317.
Chica et al., Semi-rational approaches to engineering enzyme activity: combining the benefits of directed evolution and rational design. Curr. Opi. Biotechnol., 2005, vol. 16: 378-384.
Devos et al., Practical limits of function prediction. Proteins: Structure, Function, and Genetics. 2000, vol. 41: 98-107.
Seffernick et al., Melamine deaminase and Atrazine chlorohydrolase: 98 percent identical but functionally different. J. Bacteriol.,2001, vol. 183 (8): 2405-2410.
Sen et al., Developments in directed evolution for improving enzyme functions. Appl. Biochem. Biotechnol., 2007, vol. 143: 212-223.
Whisstock et al., Prediction of protein function from protein sequence. Q. Rev. Biophysics., 2003, vol. 36 (3): 307-340.
Witkowski et al., Conversion of b-ketoacyl synthase to a Malonyl Decarboxylase by replacement of the active cysteine with glutamine. Biochemistry, 1999, vol. 38: 11643-11650.
Amidjojo et al., 2005, “Asymmetric Synthesis of Tert-butyl (3R, 5S)6-chloro-dihydroxyhexanoate withLactobacillus kefir,” Appl Microbiol Biotechnol., 69:9-15.
Bisel et al., 2007, “Stereochemical clarification of the enzyme-catalysed reduction of 2-acetylchromen-4-one,”Tetrahedron Asymmetry, 18(9):1142-1144.
Bradshaw et al., 1992, “Lactobacillus kefirAlcohol Dehydrogenase: A Useful Catalyst for Synthesis,”J. Org. Chem. 57(5):1532-1536.
Breyer-Pfaff et al., 1999, “High-affinity Stereoselective Reduction of the Enantiomers of Ketotifen and of Ketonic Nortriptyline Metabolites by Aldo-Keto Reductases from Human Liver,”Biochem. Pharmacol., 59:249-260.
Cha et al., 2002, “Stereochemical control in diastereoselective reduction of α-substituted-β-ketoesters using a reductase purified fromKluyveromyces marxianus,” Biotechnol. Lett, 24:1695-1698.
Database EPO Proteins, Apr. 2007, “Sequence 4 from Patent WO2007012428,” XP002488479, retrieved from EBI Accession No. EPOP:CS539287, Database Accession No. CS539287.
Daussmann et al., 2006, “Oxidoreductases and Hydroxynitrilase Lyases: Complementary Enzymatic Technologies for Chiral Alcohols,”Eng Life Sci., 6(2):125-129.
Fuganti et al., 1993, “Microbial Generation of (2R,3S)- and (2S,35)-Ethyl 2-Benzamidomethy1-3-hydroxybutyrate, a Key Intermediate in the Synthesis of (3S,1′R)-3-(1′-Hydroxyethyl)azetidin-2-one,”J Chem. Soc. Perkin Trans. 1:2247-2249.
Genbank Accession No. 1NXQ—A, Feb. 11, 2003.
Genbank Accession No. AJ544275, Feb. 5, 2010.
Genbank Accession No. AAP94029, Apr. 1, 2004.
Genbank Accession No. BAA24528.1, Jan. 28, 1998.
Genbank Accession No. CAD66648, Feb. 17, 2003.
Genbank Accession No. JC7338, Jun. 3, 2002.
Genbank Accession No. NP010159.1, Jun. 16, 2008.
Genbank Accession No. P41747, May 5, 2009.
Genbank Accession No. Q07551, Nov. 28, 2006.
Ghosh et al., 1993, “Cyclic Sulfolanes as Novel and High Affinity P2Ligands for HIV-1 Protease Inhibitors,”J. Med. Chem., 36(7):924-927.
Ghosh et al., 1994, “The Development of Cyclic Sulfolanes as Novel and High-Affinity P2Ligands for HIV-1 Protease Inhibitors,”J. Med. Chem. 37:1177-1188.
Goldberg et al., 2007, “Biocatalytic ketone reduction—a powerful tool for the production of chiral alcohols-part I: processes with isolated enzymes,”Appl Microbiol Biotechnol, 76(2):237-248.
Gröger et al., 2004, “Preparative asymmetric reduction of ketones in a biphasic medium with an (S)-alcohol dehydrogenase under in situ-cofactor-recycling with a formate dehydrogenase,”Tetrahedron60:633-640.
Hönig et al., 1994, “Enzymatic Resolutions of Heterocyclic Alcohols,”Biocatalysis9:61-69.
Hummel et al., 1989, “Dehydrogenases for the synthesis of chiral compounds,”Eur. J. Biochem. 184:1-13.
Hummel, 1990, “Reduction of acetophenone toR(+)-phenylethanol by a new alcohol dehydrogenase fromLactobacillus kefir,” Appl Microbiol Biotechnol, 34(1):15-19.
Hummel, 1999, “Large-scale applications of NAD(P)-dependent oxidoreductases: recent developments,”Trends Biotechnol. 17(12):487-492.
Jones et al., 1981, “Enzymes in organic syntheses. 19.1Evaluation of the stereoselectivities of horse liver alcohol dehydrogenase; catalyzed oxidoreductions of hydroxy- and ketothiolanes, -thianes, and -thiepanes,”Can. J. Chem., 59:1574-1579.
Jörnvall et al., 1995, “Short-Chain Dehydrogenase/Reductases (SDR),”Biochemistry34(18):6003-6013.
Kallberg et al., 2002, “Short-chain dehydrogenase/reductase (SDR) relationships: A large family with eight clusters common to human, animal, and plant genomes,”Protein Sci. 11(3):636-641.
Kallberg et al., 2002, “Short-chain dehydrogenases/reductases (SDRs) Coenzyme-based functional assignments in completed genomes,”Eur. J. Biochem. 269:4409-4417.
Niefind et al., 2003, “The Crystal Structure of R-specific Alcohol Dehydrogenase fromLactobacillus brevisSuggests the Structural Basis of its Metal Depende
Huisman Gjalt
Jenne Stephane J.
Lalonde James
Liang Jack
Mundorff Emily
Codexis, Inc.
Raghu Ganapa Thirama
LandOfFree
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