Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of... – Method of detaching cells – digesting tissue or establishing...
Reexamination Certificate
2007-03-06
2007-03-06
Lankford, Leon B (Department: 1651)
Chemistry: molecular biology and microbiology
Animal cell, per se ; composition thereof; process of...
Method of detaching cells, digesting tissue or establishing...
C435S366000, C435S371000
Reexamination Certificate
active
10478830
ABSTRACT:
The present invention relates to a method for inducing the differentiation of mammals' embryonic stem cells into keratinocytes, comprising the following steps of:isolating an extracellular matrix secreted by at least one mammals' cell type,cultivating mammals' embryonic stem cells in parallel in an undifferentiated condition in an appropriate culture medium in the presence of LIF,seeding the embryonic stem cells as a monolayer on said extracellular matrix,cultivating the thus seeded embryonic stem cells in the absence of LIF for a period of time sufficient for their differentiation into keratinocytes, andcollecting the thus obtained keratinocytes.
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Aberdam Daniel
Coraux Christelle
INSERM
Lankford Leon B
Merchant & Gould P.C.
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