Keratinocyte megacolonies, and assays for growth factors

Details Keratinocyte megacolonies, and assays for growth factors Keratinocyte megacolonies, and assays for growth factors

1986-08-21

1990-04-17

Warden, Robert J.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving viable micro-organism

4382402, 436 63, C12Q 102, G01N 3348

Patent

active

049180029

ABSTRACT:
Compounds are assayed for ability to promote epidermal cell growth and migration. The assay methods feature growing "megacolonies", which are large colonies having a surface area greater than 1 cm.sup.2, and measuring growth during the linear growth phase, or measuring radial expansion during the exponential growth phase. These measurment allow comparison of growth factors and provide good estimates of the biological activity of such factors. A method of producing megacolonies and the megacolonies so produced are also disclosed.

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