KDO aldolase

Details KDO aldolase KDO aldolase

1994-10-25

1996-12-17

Wityshyn, Michael G.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Lyase

4352521, 435822, C12N 986, C12N 120

Patent

active

055852610

ABSTRACT:
Aureobacterium barkerei strain KDO-37-2 (ATCC 49977) and KDO aldolase (EC 4.1.2.23) isolated therefrom are disclosed. The KDO aldolase is further disclosed to have a broad substrate specificity with respect to its reverse reaction, i.e. the condensation of aldoses with pyruvate to form a wide range of 2-keto-3-deoxy-onic acids, including 2-keto-3-deoxy-nonulosonic acid, 2-keto-3-deoxy-octulosonic acid, 2-keto-3-deoxy-heptulosonic acid, and 2-keto-3-deoxy-hexulosonic acid. In particular, 3-deoxy-D-manno-2-octulosonic acid (D-KDO), a vital component of lipopolysaccharides found in the bacterial outer membrane may be synthesized from D-arabinose and pyruvate in 67% yield.

REFERENCES:
patent: 5162513 (1992-11-01), Wong et al.
patent: 5358859 (1994-10-01), Wong et al.
Knappmann et al. (1990) "Localization of the Enzyme 3-Deoxy-D-manno-2-Octulosonic Acid Aldolase", Appl. Microbiol. Biotechnol. , 33(3), 324-329.
Sugai et al. (1993) "Synthesis of 3-Deoxy-D-manno-2-Octulosonic Acid (KDO) and Its Analogs Based on KDO Aldolase-Catalyzed Reactions", J. Am. Chem. Soc., 115(2), 413-421.
Hershberger, et al., "Chemistry and Metabolism of 3-Deoxy-D-Mannooctullosonic Acid", J. of Biol. Chem., 243:1585-1588 (1968).
Ghalambor, et al., "The Biosynthesis of Cell Wall Lipopolysaccharide(in) Escherichia coli", J. of Biol. Chem., 241:3222-3227 (1966).
Ghalambor, et al., "The Biosynthesis of Cell Wall Lipopolysaccharide in Escherichia coli", J. of Biol. Chem., 241:3209-3215 (1966).
Bednarski, et al., "Synthesis of 3-Deoxy-D-Manno-2-Octulosonate-8-Phosphate (KDO-8-P) From D-Arabinose: Generation of D-Arabinose 5-Phosphate Using Hexokinase", Tet. Letters, 29:427-430 (1988).

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