Jojoba wax biosynthesis gene

Details Jojoba wax biosynthesis gene Jojoba wax biosynthesis gene

1993-05-20

1995-08-29

Fox, David T.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

435 712, 435134, 4351723, 4352404, 536 236, 536 232, 800200, 800205, 800255, 800DIG17, C12P 104, C12P 764, A01M 100, C12N 1505

Patent

active

054459478

ABSTRACT:
By this invention, a partially purified fatty acyl-CoA: fatty alcohol acyltransferase (wax synthase) is provided, wherein said protein is active in the formation of a wax ester from fatty alcohol and fatty acyl substrates. Of special interest is a jojoba embryo wax synthase having an apparent molecular mass of approximately 57 kD. Also considered are amino acid and nucleic acid sequences obtainable from wax synthase proteins and the use of such sequences to provide transgenic host cells capable of producing wax esters.

REFERENCES:
patent: 4826767 (1989-05-01), Hansen et al.
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Anderson, et al., "Characterization of Enzymes Involved In in Biosynthesis of Long Chain Liquid Waxes of Jojoba (Simmondsia chinensis)," Plant Physiology (1992) 99:77 abstract.
Kolattukudy and Rogers, "Acyl-CoA Reductase and Acyl-CoA: Fatty Alcohol Acyl Transferase in the Microsomal Preparation From The Bovine Meibomian Gland," J. of Lipid Research (1986) 27:404-411.
Pollard, et al., "Studies on Biosynthesis of Waxes by Developing Seed. II. The Demonstration of Wax Biosynthesis by Cell-Free Homogenates," Lipids (1979) 14(7):651-662.
Pushnik, et al., "Characterization of the Biosynthetic Pathway For Formation of Liquid Wax in Jojoba," abstract The Southwest Consortium Fourth Annual Meeting, Feb. 7, 1989.
Wilder, G. and Hallick, R., "Wax Ester Biosynthesis in Euglena gracilis," abstract The Southwest Consortium Fifth Annual Meeting, Apr. 22-24, 1990.
Wu, et al., "Studies of Biosynthesis of Waxes by Developing Jojoba Seed: III. Biosynthesis of Wax Esters from Acyl-Coa and Long Chain Alcohols," Lipids (1981) 16(12):897-902.
Yamauchi, et al., "Purification and Characterization of Acyl Coenzyme A: Alcohol Acyltransferase of Neurospora sp.," Agric. Biol. Chem. (1989) 53(6):1551-1556.

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