Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1994-07-29
1998-03-17
Knode, Marian C.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 74, 435 772, 436518, G01N 33567, G01N 33573, G01N 3353, G01N 33543
Patent
active
057285365
ABSTRACT:
The present invention is based on the discovery that a critical step in the cellular response to several cytokines is the activation (i.e. tyrosine phosphorylation) of a member of the Jak kinase family. In particular, several cytokines whose activity is mediated by the activation of Jak2 kinase are identified. The present invention provides novel methods for regulating the cellular response to these cytokines by inhibiting or enhancing the Jak kinase activity which mediates the response. Assays for identifying inhibitors of Jak kinase activity or cytokine-induced Jak kinase activation useful in the methods of the invention are also provided. Antibodies raised against peptide fragments of Jak1, Jak2, and Tyk2 kinase capable of specifically binding to these Jak kinases without interfering with kinase activity are also provided. In addition, the complete DNA coding sequence and amino acid structure of Jak2 kinase is provided by the invention.
REFERENCES:
patent: 5441868 (1995-08-01), Lin
patent: 5470952 (1995-11-01), Stahl et al.
Nakajima et al., Interleukin-6 Signals Activating junB and Tls1 1 Gene Transcription in a B-Cell Hybridoma, Molecule and Cellular Biology 11(3):1409-1418, 1991.
Bird, T. et al., "Evidence That MAP (Mitogen-Activated Protein) Kinase Activation May Be A Necessary But Not Sufficient Signal For A Restricted Subset Of Responses In IL-1-Treated Epidermoid Cells," Cytokine 4(6):429-440 (Nov. 1993).
Campbell, G.S. et al., "Evidence for Involvement of the Growth Hormone Receptor-associated Tyrosine Kinase in Actions of Growth Hormone," J. Biol. Chem. 268 (10):7427-7434 (Apr. 5, 1993).
Carroll, M.P. et al., "Interleukin-3 and Granulocyte-Macrophage Colony-stimulating Factor Mediate Rapid Phosphorylation and activation of Cytosolic c-raf," J. Biol. Chem. 265 (32):19812-19817 (Nov. 15, 1990).
Carroll, M.P. et al., "Erythropoietin Induces Raf-1 Activation and Raf-1 Is Required for Erythropoietin-mediated Proliferation," J. Biol. Chem. 266 (23):14964-14969 (Aug. 15, 1991).
Cleveland, J.L. et al., "Tyrosine Kinase Oncogenes Abrogate Interleukin-3 Dependence of Murine Myeloid Cells through Signaling Pathways Involving c-myc: Conditional Regulation of c-myc Transcription by Temperature-Sensitive v-abl," Mol. and Cell. Biol. 9 (12):5685-5695 (Dec. 1989).
Dusanter-Fourt, I. et al., "Erythropoietin Induces the Tyrosine Phosphorylation of Its Own Receptor in Human Erythropoietin-responsive Cells," J. Biol. Chem. 267 (15):10670-10675 (May 25, 1992).
Edgington, S.M., "Molecular Crosstalk: Will virology and growth-factor research aid cytokine drug discovery?" Bio/Technol. 11:465-468 (Apr. 1993).
Firmbach-Kraft, I. et al., "tyk2, prototype of a novel class of non-receptor tyrosine kinase genes," Oncogene 5:1329-1336 (3 May 1990).
Fu, X-Y., "A Transcription Factor with SH2 and SH3 Domains Is Directly Activated by an Interferon .alpha.-Induced Cytoplasmic Protein Tyrosine Kinase(s)," Cell 70:323-335 (Jul. 24, 1992).
Fung, M.R. et al., "A Tyrosine Kinase Physically Associates With The .beta.-Subunit Of The Human IL-2 Receptor," J. Immunol. 147 (4):1253-1260 (Aug. 15, 1991).
Hanks, S.K. et al., "The Protein Kinase Family: Conserved Features and Deduced Phytogeny of the Catalytic Domains," Science 241:42-52 (1 Jul. 1988).
Harpur, A.G. et al., "JAK2, a third member of the JAK family of protein tyrosine kinases," Oncogene 7:1347-1353 (1992).
Howard, O.M.Z. et al., "Characterization of a class 3 tyrosine kinase," Oncogene 7:895-900 (1992).
Hunter, T., "A Thousand and One Protein Kinases," Cell 50:823-829 (Sep. 11, 1987).
Ihle, J.N., "Interleukin-3 and Hematopoiesis," Interleukins: Mol. Biol. and Immunol. 51:65-106.
Kishimoto, T. (ed.) Basel, Karger (1992).
Isfort, R.J. et al., "Interleukin 3 binds to a 140-kDa phosphotyrosine-containing cell surface protein," Proc. Natl. Acad. Sci. USA 85:7982-7986 (Nov. 1988).
Koch, C.A. et al., "SH2 and SH3 Domains: Elements That Control Interactions of Cytoplasmic Signaling Proteins," Science 252:668-674 (3 May 1991).
Linnekin, D. et al., "Signal transduction of human interleukin 3 and granulocyte-macrophage colony-stimulating factor through serine and tyrosine phosphorylation," J. Biochem 271:317-324 (1990).
Linnekin, D. et al., "Association of the erythropoietin receptor with protein tyrosine kinase activity," Proc. Natl. Acad. Sci. USA 89:6237-6241 (Jul. 1992).
Mano, H. et al., "Expression of a novel form of Tec kinase in hematopoietic cells and mapping of the gene to chromosome 5 near Kit," Oncogene 8:417-424 (1993).
Metcalf, D., "The molecular control of cell division, differentiation commitment and maturation in haemopietic cells," Nature 339:27-30 (4 May 1989).
Miura, O. et al., "Induction of Tyrosine Phosphorylation by the Erythropoietin Receptor Correlates with Mitogenesis," Mol. and Cell. Biol. 11 (10):4895-4902 (Oct. 1991).
Miyajima, A. et al., "Cytokine Receptors and Signal Transduction," Annu. Rev. Immunol. 10:295-331 (1992).
Morla, A.O. et al., "Hematopoietic Growth Factors Activate the Tyrosine Phosphorylation of Distinct Sets of Proteins in Interleukin-3-Dependent Murine Cell Lines," Mol. and Cell. Biol. 8(5):2214-2218 (May 1988).
Partanen, J. et al., "Putative tyrosine kinases expressed in K-562 human Leukemia cells," Proc. Natl. Acad. Sci. USA 87:8913-8917 (Nov. 1990).
Pellegrini, S. et al., "Use of a Selectable Marker Regulated by Alpha Interferon To Obtain Mutations in the Signaling Pathway," Mol. and Cell. Biol. 9(11):4605-4612 (Nov. 1989).
Pritchard, M.A. et al., "Two members of the JAK family of protein tyrosine kinases map to Chromosomes 1p31.3 and 9p24," Mammalian Genome 3:36-38 (1992).
Quelle, F.W. et al., "Proliferative Action of Erythropoietin Is Associated with Rapid Protein Tyrosine Phosphorylation in Responsive B6SUt.EP Cells," J. Biol. Chem. 266 (1):609-614 (Jan. 5, 1991).
Schindler, C. et al., "Interferon-Dependent Tyresine Phosphorytation of a Latent Cytoplasmic Transcription Factor," Science 257:809-813 (7 Aug. 1992).
Shuai, K. et al., "Activation of Transcription by IFN-.gamma.: Tyrosine Phosphorylation of a 91-kD DNA Binding Protein," Science 258:1808-1812 (11 Dec. 1992).
Sorensen, P. et al., "Interleukin-3 Stimulates the Tyrosine Phosphorylation of the 140-Kilodalton Interteukin-3 Receptor," J. Biol. Chem. 264 (32):19253-19258 (Nov. 15, 1989).
Spangler, R. et al., "Erythropoietin Increases c-myc mRNA by a Protein Kinase C-dependent Pathway," J. Biol. Chem. 266 (2):681-684 (Jan. 15, 1991).
Torigoe, T. et al., "Interleukin-3 Regulates the Activity of the LYN Protein-Tyrosine Kinase in Myeloid Committed Leukemic Cell Lines," Blood 80 (3):617-624 (Aug. 1, 1992).
Turner, B. et al., "Interleukin 2 induces tyrosine phosphorylation and activation of p72-74 Raf-1 kinase in a T-cell line," Proc. Natl. Acad. Sci USA 88:1227-1231 (Feb. 1991).
Uttrich, A. et al., "Signal Transduction by Receptors with Tyrosine Kinase Activity," Cell 61:203-212 (Apr. 20, 1990).
Valezquez, L. et al., "A Protein Tyrosine Kinase in the Interferon .alpha./.beta. Signaling Pathway," Cell 70:313-322 (Jul. 24, 1992).
Wang, X. et al., "Growth Hormone-promoted Tyrosyl Phosphorytation of a 121-kDa Growth Hormone Receptor-associated Protein," J. Biol. Chem. 268 (5):3573-3579 (Feb. 15, 1993).
Wilks, A.F., "Two putative protein-tyrosine kinases identified by application of the polymerase chain reaction," Proc. Natl. Acad. Sci. USA 86:1603-1607 (Mar. 1989).
Wilks, A.F., "Structure And Function Of The Protein Tyrosine Kinases," Prog. in Growth Factor Res. 2:97-111 (1990).
Wilks, A.F., "Cloning Members of Protein-Tyrosine Kinase Family Using Polymerase Chain Reaction," Meth. in Enzylmol. 200:533-546 (1991).
Wilks, A. et al., "Two Novel Protein-Tyrosine Kinases, Each with a Second Phosphotransferase-Related Catalytic Domain, Define a New Class of Protein Kinase," Mol. and Cell. Biol. 11 (4):2057-2065 (Apr. 1991).
Yarden, Y. et al., "Growth Factor Receptor Tyrosine Kinases," Ann. Rev. Biochem. 57:443-478 (1988).
Yoshimura, A. et al., "Point mutation in the exoplasmic domain of the erythropoietin receptor resulting in hormone-independent activation and tumorige
Ihle James N.
Quelle Frederick W.
Silvennoinen Ollie
Witthuhn Bruce A.
Knode Marian C.
St. Jude Children's Research Hospital
Wortman Donna C.
LandOfFree
Jak kinases and regulation of Cytokine signal transduction does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Jak kinases and regulation of Cytokine signal transduction, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Jak kinases and regulation of Cytokine signal transduction will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-956520