IspA from Streptococcus pneumoniae

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

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4352523, 4353201, 435325, 536 231, 536 235, 536 232, 536 234, 536 237, C12P 2106

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active

061300691

ABSTRACT:
The invention provides ispA polypeptides and polynucleotides encoding ispA polypeptides and methods for producing such polypeptides by recombinant techniques. Also provided are methods for utilizing ispA polypeptides to screen for antibacterial compounds.

REFERENCES:
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Koyama, T., "Micrococcus luteus DNA for farnesyl diphosphate synthase, complete cds", GenBank Submission, Accession No. AB003187, Direct Sumbission, Mar. 25, 1998.
Tomb, et al., "Helicobacter pylori 26695 section 80 of 134 of the complete genome", GenBank Submission, Accession No. AE000602, Aug. 6, 1997.
Ogura, K., "B. stearothermophilus DNA for farnesyl diphosphate synthase", GenBank Submission, Accession No. D13293, Direct Submission, Aug. 6, 1993.
Nakane, et al., "Crystallization and Preliminary X-ray Diffraction Studies of Bacillus stearothermophilus farnesyl Disphosphate Synthase Expressed in Escherichia coli", J. Mol. Biol, vol. 233, pp. 787-788, (1993).
Koyama, et al., "Identification of Significant Residues in the Substrate Binding Site of Bacillus stearothermophilus Farnesyl Diphosphate Synthase", Biochemistry, vol. 35, pp. 9533-9538, (1996).
Fujisaki, et al., "Coning and Nucleotide Sequence of the ispA Gene Responsible for Farnesyl Diphosphate Synthase Activity in Escherichia coli", J. Biochem., vol. 108, pp. 995-1000, (1990).
Tarshis, et al., "Crystal Structure of Recombinant Farnesyl Diphosphate Synthase at 2.6-.ANG. Resolution", Biochemistry, vol. 33, pp. 10871-10877, (1994).
Siomoin, et al., "Identification and characterization of ispA, a Shigella flexneri chromosomal gene essential for normal in vivo cell division and intercellular spreading", Molecular Microbiology, vol. 19, No. 3, pp 599-609, (1996).

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