Isozymes of lacrimator component synthase and gene encoding...

Details Isozymes of lacrimator component synthase and gene encoding... Isozymes of lacrimator component synthase and gene encoding...

2008-05-13

2008-05-13

Prouty, Rebecca E. (Department: 1652)

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Lyase

C435S183000, C536S023200

Reexamination Certificate

active

07371554

ABSTRACT:
It is an object of the present invention to provide isozymes of the lachrymatory factor producing enzyme, the amino acid sequences of these isozymes and a gene that codes for these amino acid sequences, and the present invention relates to three types of isozymes of the lachrymatory factor producing enzyme that contributes to the production of the lachrymatory factor that is present in onions and the like, amino acid sequences indicated by SEQ ID Nos. 1 to 3 which constitute the proteins or polypeptides of these isozymes, DNA indicated by SEQ ID Nos. 4 and 5 which contains base sequences that code for the abovementioned proteins or polypeptides, a method of producing the abovementioned isozymes, a recombinant vector which contains the abovementioned DNA, a transformant formed by transforming a host cell with the abovementioned recombinant vector, a method of producing proteins or polypeptides that have lachrymatory factor producing enzyme activity by culturing the abovementioned host cell, and anti-sense RNA which has a base sequence that is complementary to that of the mRNA corresponding to the abovementioned DNA.

REFERENCES:
patent: 10-295373 (1998-11-01), None
Van Damme et al. “Isolation and characterization of allinase cDNA clones from garlic (Allium sativum L.) and related species”, Eur J Biochem. Oct. 15, 1992;209(2):751-7.
Block E. et al., The organosulfer chemistry of the genus Allium: Implications for the oranic chemistry of sulfer. Angewandte Chemie International Edition in English, 1992, 31(9), pp. 1135-1178.
Krest I. et al., Cysteine sulfoxides and alliinase activity of some allium species. Journal of Agricultural and Food Chemistry, Aug. 2000, 48(8), pp. 3753-3760.
Kopsell D.E. et al., Changes in the S-alk(en)yl cysteine sulfoxides and their biosynthetic intermediates during onionstorage. Journal of the American Society for Horticultural Science, Mar. 1999, 124 (2), pp. 177-183.
Block, Eric. “The Organosulfur Chemistry of the Genus Allium—Implications for the Organic Chemistry of Sulfur”, Angew. Chem. Int. Ed. Engl., vol. 31, No. 9, pp. 1135-1178 1992.
Krest, I. et al. “Cysteine Sulfoxides and Alliinase Activity of Some Allium Species”, J. Agric. Food Chem, vol. 48, No. 8, pp. 3753-3760 2000.
Kopsell, David E. et al. “Changes in the S-alk(en)yl Cysteine Sulfoxides and their Biosynthetic Intermediates during Onion Storage”, J. Amer. Soc. Hort. Sci., vol. 124, No. 2, pp. 177-183 1999.

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