Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Oxidoreductase
Reexamination Certificate
2006-05-02
2006-05-02
Witz, Jean C. (Department: 1651)
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Oxidoreductase
C435S183000, C424S094400
Reexamination Certificate
active
07037697
ABSTRACT:
The invention relates to a novel purified isozyme of an autoclavable superoxide dismutase extracted from the plantPotentilla atrosanguineaLodd. Var.areyrophylla,said isozyme having the following characteristics, O2−scavenging activity remains same before and after autoclaving; scavenges O2−from sub-zero temperature of −20° C. to high temperature of +80° C.; O2−scavenging activity at 25° C. for 30 days without adding any stabilizing agent such as polyols or sugars; O2−scavenging activity in the presence of saline (0.9% sodium chloride) to 61.8% of the control (without 0.9% sodium chloride), stable at 4° C. for at least 8 months; contamination free and infection free from any living micro- and/or macro-organism after autoclaving; possesses temperature optima at 0° C.; possesses a molecular weight of 33 kD under non-denaturating conditions; possesses a molecular weight of 36 kD under denaturating conditions; has clear peaks in UV range at 268 and 275 nm; has an enzyme turnover number of 19.53×104% per nmol per min at 0° C.; and requires Cu/Zn as a co-factor, method for the preparation of the purified isozyme of autoclavable superoxide dismutase and formulations containing the said autoclavable superoxide dismutase.
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Beuno et al., Peroxisomal copper, zinc super oxide dismutase, characterization of the isoenzyme from watermelon cotyledons (1985)Plant Physiol.vol. 108, pp. 1151-1160.
Gupta et al., Increased resistance to oxidative stress in transgenic plants that overexpress chloroplastic Cu/Zn superoxide dimutase (1993)PNSA,USA, vol.90, pp. 1629-1633.
Ahuja Paramvir Singh
Kumar Sanjay
Sahoo Rashmita
Council of Scientific & Industrial Research
Foley & Lardner LLP
Hanley Susan
Witz Jean C.
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