Isotopically-labeled proteome standards

Details Isotopically-labeled proteome standards Isotopically-labeled proteome standards

2011-05-10

2011-05-10

Desai, Anand U (Department: 1656)

Chemistry: analytical and immunological testing

Peptide, protein or amino acid

C436S173000, C435S041000

Reexamination Certificate

active

07939331

ABSTRACT:
The invention provides methods for quantifying biomolecules, such as polypeptides in mass spectrometric analysis. The methods include use of a biomolecule standard having at least one atomic isotope different than that of the naturally occurring isotopes in the biomolecule of interest. Methods of the present invention also include methods for quantifying biomolecules where the copy biomolecule standard is made by expressing the biomolecule using a recombinant cell. Further included are the biomolecule standards themselves, method for making such standards, kits, systems, reagents, and engineered cells relating to the use of biomolecule standards in mass spectrometric analysis.

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