Isothermal transcription based amplification assay for the detec

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

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435 913, 536 231, 536 243, 536 2433, C12Q 168, C12P 1934, C07H 2104

Patent

active

060935421

ABSTRACT:
An isothermal transcription based amplification assay for MDC RNA uses primer combinations for sequences within the MDC gene. A quantitative control uses a mutant RNA for comparison.

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patent: 5554517 (1996-09-01), Davey et al.
Shaffer et al. Amplification, detection, and automated sequencing of Gibbon interleuin-2 mRNA by Thermus aquaticus DNA polymerase reverse transcription and polymerase chain reaction. Anal. Bioch. vol. 190;292-296, Oct. 1990.
R. Pal et al., Science, 278:695-698, 1997.

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