Isothermal strand displacement nucleic acid amplification

Details Isothermal strand displacement nucleic acid amplification Isothermal strand displacement nucleic acid amplification

2006-03-07

2006-03-07

Sitton, Jehanne (Department: 1634)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

C435S091200, C435S006120, C536S023100, C536S024330

Reissue Patent

active

RE039007

ABSTRACT:
Methods for amplifying target nucleic acid sequences using a nucleic acid polymerase lacking 5′ exonuclease activity and a set of oligonucleotide primers. Preferably, a primer array is used. The primer array contains two sets of primers. One set contains at least two complementary primers. The other set contains at least two sense primers. Using the described methods amplification can be carried out under essentially constant environmental conditions without the requirement for exonuclease activity or restriction endonu- clease activity.

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